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• W2072061966 abstract "The established human histiocytic lymphoma cell line U-937 spontaneously produced a factor with biological activity similar to that ascribed to interleukin 1 (IL1). Actually, supernatants from U-937 cells promoted proliferation of thymocytes initiated by concanavalin A (Con A) and replaced the requirement of accessory cells for activation of highly purified circulating T lymphocytes induced by Con A. Phorbol myristate acetate (PMA) significantly increased the titers of the helper factor produced by U-937 cells as compared to that secreted by non-PMA-treated U-937 cells or PMA-stimulated P388D1 murine macrophage tumor cells. Generally U-937 cells did not secrete detectable IL1 activity during the first 24-48 h of culture. However, after this initial period the level of IL 1 activity increased and reached a maximum at 5-6 days of culture. Finally, the helper factor released by U-937 cells had an apparent mol. wt. of 12000-15000 as determined by Sephadex G-100 chromatography and lacked interleukin 2 activity as shown by its inability to support growth of IL 2-addicted T cell lines. To our knowledge this is the first report of an established human cell line capable of producing ILL 1." @default.
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• W2072061966 date "1982-01-01" @default.
• W2072061966 modified "2023-10-03" @default.
• W2072061966 title "Accessory function of human tumor cell lines I Production of interleukin 1 by the human histiocytic lymphoma cell line U-937" @default.
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• W2072061966 doi "https://doi.org/10.1002/eji.1830121018" @default.
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