Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072072743> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2072072743 endingPage "1545" @default.
- W2072072743 startingPage "1537" @default.
- W2072072743 abstract "Pituitary tumor transforming gene 1 (PTTG1), recently cloned from human testis, is a potent oncogene that is expressed in most tumors. However, assessment of its potential value as a prognostic marker is dependent on the development of a suitable antibody. We have developed a rabbit polyclonal antibody, SK601, that is highly specific for the PTTG1 gene product using recombinant PTTG1 protein (24 kD) containing an N-terminal His 6 tag as the immunogen. The antiserum is capable of detecting recombinant PTTG1 protein in ELISA assays at a titer of 1:100,000. Use of the antibody as the probe in Western blotting analyses revealed a single band with the anticipated relative molecular weights of 52 kD from E. coli expressing the GST-PTTG1 recombinant protein, and 56 kD from COS-7 cells transfected with the PTTG1-GFP chimeric construct. A single band with a relative molecular weight of 28 kD was observed in extract of COS-7 cells transfected with PTTG1 cDNA. The antiserum immunoprecipitated a protein of relative molecular weight of 56 kD from the extracts of COS-7 cells transfected with the PTTG1-GFP chimeric construct. Immunohistochemical analysis of COS-7 cells transfected with this construct confirmed that the antibody detected and was specific for expressing the PTTG1-GFP recombinant protein. Screening of various normal human tissues (testis, ovary, and breast) by immunohistochemistry indicated that these tissues did not exhibit staining with the exception of testis, a tissue that had previously been shown to express PTTG1 mRNA. In contrast all of the tumor tissues (testicular tumor, ovarian tumor, and breast tumor) that were assessed exhibited intense staining. The results suggest that antiserum SK601 is highly specific for the PTTG1 protein and therefore should prove useful in further analysis of the expression and interactions of this protein, including its potential application as an immunohistochemical marker of human tumors." @default.
- W2072072743 created "2016-06-24" @default.
- W2072072743 creator A5012343353 @default.
- W2072072743 creator A5026756354 @default.
- W2072072743 creator A5052281306 @default.
- W2072072743 creator A5088508476 @default.
- W2072072743 creator A5089555322 @default.
- W2072072743 date "2001-12-01" @default.
- W2072072743 modified "2023-09-26" @default.
- W2072072743 title "Characterization of a Polyclonal Antibody to Human Pituitary Tumor Transforming Gene 1 (PTTG1) Protein" @default.
- W2072072743 cites W1965912140 @default.
- W2072072743 cites W1975957264 @default.
- W2072072743 cites W1991321447 @default.
- W2072072743 cites W2009099332 @default.
- W2072072743 cites W2029826177 @default.
- W2072072743 cites W2037060844 @default.
- W2072072743 cites W2038197766 @default.
- W2072072743 cites W2043563174 @default.
- W2072072743 cites W2058036485 @default.
- W2072072743 cites W2071649852 @default.
- W2072072743 cites W2080149096 @default.
- W2072072743 cites W2097585410 @default.
- W2072072743 cites W2146513771 @default.
- W2072072743 doi "https://doi.org/10.1177/002215540104901207" @default.
- W2072072743 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11724901" @default.
- W2072072743 hasPublicationYear "2001" @default.
- W2072072743 type Work @default.
- W2072072743 sameAs 2072072743 @default.
- W2072072743 citedByCount "16" @default.
- W2072072743 countsByYear W20720727432012 @default.
- W2072072743 countsByYear W20720727432016 @default.
- W2072072743 countsByYear W20720727432019 @default.
- W2072072743 crossrefType "journal-article" @default.
- W2072072743 hasAuthorship W2072072743A5012343353 @default.
- W2072072743 hasAuthorship W2072072743A5026756354 @default.
- W2072072743 hasAuthorship W2072072743A5052281306 @default.
- W2072072743 hasAuthorship W2072072743A5088508476 @default.
- W2072072743 hasAuthorship W2072072743A5089555322 @default.
- W2072072743 hasConcept C104317684 @default.
- W2072072743 hasConcept C123894998 @default.
- W2072072743 hasConcept C153911025 @default.
- W2072072743 hasConcept C159654299 @default.
- W2072072743 hasConcept C186852380 @default.
- W2072072743 hasConcept C187882448 @default.
- W2072072743 hasConcept C203014093 @default.
- W2072072743 hasConcept C204232928 @default.
- W2072072743 hasConcept C2776415932 @default.
- W2072072743 hasConcept C2779909307 @default.
- W2072072743 hasConcept C40767141 @default.
- W2072072743 hasConcept C54009773 @default.
- W2072072743 hasConcept C542903549 @default.
- W2072072743 hasConcept C55493867 @default.
- W2072072743 hasConcept C86803240 @default.
- W2072072743 hasConcept C91523082 @default.
- W2072072743 hasConcept C956191 @default.
- W2072072743 hasConceptScore W2072072743C104317684 @default.
- W2072072743 hasConceptScore W2072072743C123894998 @default.
- W2072072743 hasConceptScore W2072072743C153911025 @default.
- W2072072743 hasConceptScore W2072072743C159654299 @default.
- W2072072743 hasConceptScore W2072072743C186852380 @default.
- W2072072743 hasConceptScore W2072072743C187882448 @default.
- W2072072743 hasConceptScore W2072072743C203014093 @default.
- W2072072743 hasConceptScore W2072072743C204232928 @default.
- W2072072743 hasConceptScore W2072072743C2776415932 @default.
- W2072072743 hasConceptScore W2072072743C2779909307 @default.
- W2072072743 hasConceptScore W2072072743C40767141 @default.
- W2072072743 hasConceptScore W2072072743C54009773 @default.
- W2072072743 hasConceptScore W2072072743C542903549 @default.
- W2072072743 hasConceptScore W2072072743C55493867 @default.
- W2072072743 hasConceptScore W2072072743C86803240 @default.
- W2072072743 hasConceptScore W2072072743C91523082 @default.
- W2072072743 hasConceptScore W2072072743C956191 @default.
- W2072072743 hasIssue "12" @default.
- W2072072743 hasLocation W20720727431 @default.
- W2072072743 hasLocation W20720727432 @default.
- W2072072743 hasOpenAccess W2072072743 @default.
- W2072072743 hasPrimaryLocation W20720727431 @default.
- W2072072743 hasRelatedWork W2348991363 @default.
- W2072072743 hasRelatedWork W2349450117 @default.
- W2072072743 hasRelatedWork W2349455210 @default.
- W2072072743 hasRelatedWork W2359256335 @default.
- W2072072743 hasRelatedWork W2363435241 @default.
- W2072072743 hasRelatedWork W2380817849 @default.
- W2072072743 hasRelatedWork W2394322549 @default.
- W2072072743 hasRelatedWork W2394407329 @default.
- W2072072743 hasRelatedWork W2399647684 @default.
- W2072072743 hasRelatedWork W2408963792 @default.
- W2072072743 hasVolume "49" @default.
- W2072072743 isParatext "false" @default.
- W2072072743 isRetracted "false" @default.
- W2072072743 magId "2072072743" @default.
- W2072072743 workType "article" @default.