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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072225758> ?p ?o ?g. }

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• W2072225758 abstract "Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2 alpha, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have little effect when used individually. Astrocytes grown in the defined medium exhibit dramatic changes in morphological characteristics in comparison to cells grown in serum-free or serum-supplemented medium. In addition, these cells express the astrocyte-specific marker glial fibrillary acidic protein and are estimated by several criteria to be greater than 95% astrocytes." @default.
• W2072225758 created "2016-06-24" @default.
• W2072225758 creator A5080721533 @default.
• W2072225758 creator A5081730976 @default.
• W2072225758 date "1981-11-01" @default.
• W2072225758 modified "2023-10-18" @default.
• W2072225758 title "Growth of purified astrocytes in a chemically defined medium." @default.
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• W2072225758 doi "https://doi.org/10.1073/pnas.78.11.7205" @default.
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