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- W2072227693 abstract "We present evidence that Tn10 transposase promotes double strand breaks and single strand joints at Tn10 termini in vivo. Plasmids containing a shortened Tn10 element and a transposase overproducer fusion give rise, upon transposase induction, to new DNA species. The most prominent class is a circularized transposon molecule whose structure suggests that it arises from double strand breakage at the two transposon ends followed by covalent joining between the 3' and 5' ends of one of the two strands. We have used formation of the circularized transposon as a physical assay for the interaction between transposase and different mutant and wild-type termini. These experiments show that transposase protein interacts preferentially with the genetically most active termini in a way that suppresses productive interaction with weaker termini present on the same substrate molecule." @default.
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- W2072227693 date "1984-11-01" @default.
- W2072227693 modified "2023-09-25" @default.
- W2072227693 title "Transposase promotes double strand breaks and single strand joints at Tn10 termini in vivo" @default.
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- W2072227693 doi "https://doi.org/10.1016/0092-8674(84)90204-6" @default.
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