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- W2072241566 abstract "Volatile organic compounds (VOCs) in breath may serve as biomarkers of pulmonary infection or inflammation. We developed and validated a new breath sampling method for VOC analysis in ventilated patients. Breath was collected from the ventilatory circuit using cheap disposables. VOCs were identified by gas-chromatography and mass-spectrometry (GC–MS) at various minute volumes during ventilation of an artificial lung (in vitro) and ventilated patients (in vivo). Sixty-four VOCs emendated from the ventilator and tubing. Their concentrations had an inverse correlation with minute volume in in vitro experiments (median correlation coefficient: −0.61 [25–75th percentile: −0.66 to −0.43]). Forty-four of these “ventilator-associated VOCs” were also observed in vivo, without correlations with minute volume. In vivo experiments showed that only positive end-expiratory pressure influenced the concentration of breath VOCs. The sampling method was highly reproducible (median intra-class correlation 0.95 [25–75th percentile: 0.87–0.97]). In conclusion, a novel, simple and repeatable sampling method was developed and validated for capturing exhaled VOCs in ventilated patients, which could allow for large-scale breath analysis in clinical studies." @default.
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- W2072241566 date "2014-01-01" @default.
- W2072241566 modified "2023-10-17" @default.
- W2072241566 title "A simple breath sampling method in intubated and mechanically ventilated critically ill patients" @default.
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- W2072241566 doi "https://doi.org/10.1016/j.resp.2013.11.001" @default.
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