FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072274257> ?p ?o ?g. }

• W2072274257 endingPage "e220" @default.
• W2072274257 startingPage "e220" @default.
• W2072274257 abstract "Positive-strand RNA viruses are the largest genetic class of viruses and include many serious human pathogens. All positive-strand RNA viruses replicate their genomes in association with intracellular membrane rearrangements such as single- or double-membrane vesicles. However, the exact sites of RNA synthesis and crucial topological relationships between relevant membranes, vesicle interiors, surrounding lumens, and cytoplasm generally are poorly defined. We applied electron microscope tomography and complementary approaches to flock house virus (FHV)–infected Drosophila cells to provide the first 3-D analysis of such replication complexes. The sole FHV RNA replication factor, protein A, and FHV-specific 5-bromouridine 5'-triphosphate incorporation localized between inner and outer mitochondrial membranes inside ∼50-nm vesicles (spherules), which thus are FHV-induced compartments for viral RNA synthesis. All such FHV spherules were outer mitochondrial membrane invaginations with interiors connected to the cytoplasm by a necked channel of ∼10-nm diameter, which is sufficient for ribonucleotide import and product RNA export. Tomographic, biochemical, and other results imply that FHV spherules contain, on average, three RNA replication intermediates and an interior shell of ∼100 membrane-spanning, self-interacting protein As. The results identify spherules as the site of protein A and nascent RNA accumulation and define spherule topology, dimensions, and stoichiometry to reveal the nature and many details of the organization and function of the FHV RNA replication complex. The resulting insights appear relevant to many other positive-strand RNA viruses and support recently proposed structural and likely evolutionary parallels with retrovirus and double-stranded RNA virus virions." @default.
• W2072274257 created "2016-06-24" @default.
• W2072274257 creator A5026899432 @default.
• W2072274257 creator A5033306591 @default.
• W2072274257 creator A5049853144 @default.
• W2072274257 creator A5051156147 @default.
• W2072274257 creator A5060956579 @default.
• W2072274257 date "2007-08-14" @default.
• W2072274257 modified "2023-10-03" @default.
• W2072274257 title "Three-Dimensional Analysis of a Viral RNA Replication Complex Reveals a Virus-Induced Mini-Organelle" @default.
• W2072274257 cites W1500508967 @default.
• W2072274257 cites W1519916614 @default.
• W2072274257 cites W1555271774 @default.
• W2072274257 cites W1596323100 @default.
• W2072274257 cites W1638707894 @default.
• W2072274257 cites W1820380997 @default.
• W2072274257 cites W1921883182 @default.
• W2072274257 cites W1933124086 @default.
• W2072274257 cites W1956141565 @default.
• W2072274257 cites W1967516118 @default.
• W2072274257 cites W1970093056 @default.
• W2072274257 cites W1973933701 @default.
• W2072274257 cites W1975227291 @default.
• W2072274257 cites W1978512485 @default.
• W2072274257 cites W1978930640 @default.
• W2072274257 cites W1979039733 @default.
• W2072274257 cites W1982254939 @default.
• W2072274257 cites W1988110565 @default.
• W2072274257 cites W1994582860 @default.
• W2072274257 cites W2003298818 @default.
• W2072274257 cites W2006100734 @default.
• W2072274257 cites W2009321318 @default.
• W2072274257 cites W2013136727 @default.
• W2072274257 cites W2017919672 @default.
• W2072274257 cites W2018415759 @default.
• W2072274257 cites W2018986990 @default.
• W2072274257 cites W2022687771 @default.
• W2072274257 cites W2039276913 @default.
• W2072274257 cites W2042969893 @default.
• W2072274257 cites W2044036198 @default.
• W2072274257 cites W2066828546 @default.
• W2072274257 cites W2076694007 @default.
• W2072274257 cites W2079539307 @default.
• W2072274257 cites W2081483072 @default.
• W2072274257 cites W2085229930 @default.
• W2072274257 cites W2087333401 @default.
• W2072274257 cites W2092135609 @default.
• W2072274257 cites W2092333889 @default.
• W2072274257 cites W2100155449 @default.
• W2072274257 cites W2101173100 @default.
• W2072274257 cites W2102610618 @default.
• W2072274257 cites W2104765679 @default.
• W2072274257 cites W2105082304 @default.
• W2072274257 cites W2105886687 @default.
• W2072274257 cites W2107516290 @default.
• W2072274257 cites W2110671968 @default.
• W2072274257 cites W2113967550 @default.
• W2072274257 cites W2120070362 @default.
• W2072274257 cites W2123100056 @default.
• W2072274257 cites W2124771674 @default.
• W2072274257 cites W2125119690 @default.
• W2072274257 cites W2125659451 @default.
• W2072274257 cites W2132444008 @default.
• W2072274257 cites W2132520995 @default.
• W2072274257 cites W2132620892 @default.
• W2072274257 cites W2134576488 @default.
• W2072274257 cites W2135727631 @default.
• W2072274257 cites W2136452042 @default.
• W2072274257 cites W2137294277 @default.
• W2072274257 cites W2139609944 @default.
• W2072274257 cites W2140152399 @default.
• W2072274257 cites W2147313835 @default.
• W2072274257 cites W2149217385 @default.
• W2072274257 cites W2149701608 @default.
• W2072274257 cites W2153142359 @default.
• W2072274257 cites W2154107258 @default.
• W2072274257 cites W2154532083 @default.
• W2072274257 cites W2157781353 @default.
• W2072274257 cites W2162976241 @default.
• W2072274257 cites W2163165207 @default.
• W2072274257 cites W2164492221 @default.
• W2072274257 cites W2166293484 @default.
• W2072274257 cites W2177731497 @default.
• W2072274257 doi "https://doi.org/10.1371/journal.pbio.0050220" @default.
• W2072274257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1945040" @default.
• W2072274257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17696647" @default.
• W2072274257 hasPublicationYear "2007" @default.
• W2072274257 type Work @default.
• W2072274257 sameAs 2072274257 @default.
• W2072274257 citedByCount "272" @default.
• W2072274257 countsByYear W20722742572012 @default.
• W2072274257 countsByYear W20722742572013 @default.
• W2072274257 countsByYear W20722742572014 @default.
• W2072274257 countsByYear W20722742572015 @default.
• W2072274257 countsByYear W20722742572016 @default.
• W2072274257 countsByYear W20722742572017 @default.
• W2072274257 countsByYear W20722742572018 @default.
• W2072274257 countsByYear W20722742572019 @default.

• next