Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072274286> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2072274286 abstract "Expression of human interleukin 2 (IL-2) at high levels has been achieved in human cells by cotransfection and subsequent coamplification of the transfected sequences. IL-2 production by these cells reached 2700 U/ml of culture medium. This productivity was higher than the 100U/ml attained by concanavalin A (ConA) stimulated human leukaemic Jurkat, T cells. A plasmid, pSDI, containing human IL-2 cDNA and mouse dihydrofolate reductase (DHFR) cDNA was con-structed, and cotransfected with plasmid pSV2neo at a 10:1 molar ratio, into Hela (human epitheloid carcinoma) cells by the DNA-calcium phosphate coprecipitation technique. Transformants producing IL-2 were easily obtained among antibiotic G418 resistant colonies. Cells resistant to increased levels of methotrexate (MTX) secreted a large amount of IL-2 as a result of coamplification of DHFR cDNA and IL-2 cDNA. Expression of IL-2 cDNA was stimulated by removing a G-C stretch which was present between Simian Virus 40 (SV40) early promoter and IL-2 cDNA. Plas..." @default.
- W2072274286 created "2016-06-24" @default.
- W2072274286 creator A5029507076 @default.
- W2072274286 creator A5049600123 @default.
- W2072274286 creator A5054424736 @default.
- W2072274286 date "1987-01-01" @default.
- W2072274286 modified "2023-09-25" @default.
- W2072274286 title "Expression of amplified cDNA sequences encoding human interleukin 2 in human cells." @default.
- W2072274286 cites W2144634347 @default.
- W2072274286 doi "https://doi.org/10.1271/bbb1961.51.1385" @default.
- W2072274286 hasPublicationYear "1987" @default.
- W2072274286 type Work @default.
- W2072274286 sameAs 2072274286 @default.
- W2072274286 citedByCount "0" @default.
- W2072274286 crossrefType "journal-article" @default.
- W2072274286 hasAuthorship W2072274286A5029507076 @default.
- W2072274286 hasAuthorship W2072274286A5049600123 @default.
- W2072274286 hasAuthorship W2072274286A5054424736 @default.
- W2072274286 hasBestOaLocation W20722742861 @default.
- W2072274286 hasConcept C104317684 @default.
- W2072274286 hasConcept C153911025 @default.
- W2072274286 hasConcept C179464577 @default.
- W2072274286 hasConcept C185592680 @default.
- W2072274286 hasConcept C187882448 @default.
- W2072274286 hasConcept C203014093 @default.
- W2072274286 hasConcept C22744801 @default.
- W2072274286 hasConcept C2776090121 @default.
- W2072274286 hasConcept C2777366897 @default.
- W2072274286 hasConcept C2781320022 @default.
- W2072274286 hasConcept C33161422 @default.
- W2072274286 hasConcept C40767141 @default.
- W2072274286 hasConcept C54009773 @default.
- W2072274286 hasConcept C54355233 @default.
- W2072274286 hasConcept C552990157 @default.
- W2072274286 hasConcept C55493867 @default.
- W2072274286 hasConcept C81885089 @default.
- W2072274286 hasConcept C86803240 @default.
- W2072274286 hasConcept C8891405 @default.
- W2072274286 hasConceptScore W2072274286C104317684 @default.
- W2072274286 hasConceptScore W2072274286C153911025 @default.
- W2072274286 hasConceptScore W2072274286C179464577 @default.
- W2072274286 hasConceptScore W2072274286C185592680 @default.
- W2072274286 hasConceptScore W2072274286C187882448 @default.
- W2072274286 hasConceptScore W2072274286C203014093 @default.
- W2072274286 hasConceptScore W2072274286C22744801 @default.
- W2072274286 hasConceptScore W2072274286C2776090121 @default.
- W2072274286 hasConceptScore W2072274286C2777366897 @default.
- W2072274286 hasConceptScore W2072274286C2781320022 @default.
- W2072274286 hasConceptScore W2072274286C33161422 @default.
- W2072274286 hasConceptScore W2072274286C40767141 @default.
- W2072274286 hasConceptScore W2072274286C54009773 @default.
- W2072274286 hasConceptScore W2072274286C54355233 @default.
- W2072274286 hasConceptScore W2072274286C552990157 @default.
- W2072274286 hasConceptScore W2072274286C55493867 @default.
- W2072274286 hasConceptScore W2072274286C81885089 @default.
- W2072274286 hasConceptScore W2072274286C86803240 @default.
- W2072274286 hasConceptScore W2072274286C8891405 @default.
- W2072274286 hasLocation W20722742861 @default.
- W2072274286 hasOpenAccess W2072274286 @default.
- W2072274286 hasPrimaryLocation W20722742861 @default.
- W2072274286 hasRelatedWork W1952680786 @default.
- W2072274286 hasRelatedWork W2039308650 @default.
- W2072274286 hasRelatedWork W2060841491 @default.
- W2072274286 hasRelatedWork W2074435004 @default.
- W2072274286 hasRelatedWork W2089086230 @default.
- W2072274286 hasRelatedWork W2189265330 @default.
- W2072274286 hasRelatedWork W2357168565 @default.
- W2072274286 hasRelatedWork W2361098916 @default.
- W2072274286 hasRelatedWork W2375148766 @default.
- W2072274286 hasRelatedWork W2376067926 @default.
- W2072274286 hasRelatedWork W2390021201 @default.
- W2072274286 hasRelatedWork W2391418857 @default.
- W2072274286 hasRelatedWork W2403487157 @default.
- W2072274286 hasRelatedWork W2413314792 @default.
- W2072274286 hasRelatedWork W2416035794 @default.
- W2072274286 hasRelatedWork W2416204398 @default.
- W2072274286 hasRelatedWork W2416575707 @default.
- W2072274286 hasRelatedWork W3145787805 @default.
- W2072274286 hasRelatedWork W3151231603 @default.
- W2072274286 hasRelatedWork W2827640201 @default.
- W2072274286 isParatext "false" @default.
- W2072274286 isRetracted "false" @default.
- W2072274286 magId "2072274286" @default.
- W2072274286 workType "article" @default.