FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072275500> ?p ?o ?g. }

• W2072275500 endingPage "30" @default.
• W2072275500 startingPage "24" @default.
• W2072275500 abstract "An extracellular cholesterol oxidase producer, Pseudomonas aeruginosa strain PA157, was isolated by a screening method to detect 6β-hydroperoxycholest-4-en-3-one-forming cholesterol oxidase. On the basis of a putative cholesterol oxidase gene sequence in the genome sequence data of P. aeruginosa strain PAO1, the cholesterol oxidase gene from strain PA157 was cloned. The mature form of the enzyme was overexpressed in Escherichia coli cells. The overexpressed enzyme formed inclusion bodies in recombinant E. coli cells grown at 20°C and 30°C. A soluble and active PA157 enzyme was obtained when the recombinant cells were grown at 10°C. The purified enzyme was stable at pH 5.5 to 10 and was most active at pH 7.5–8.0, showing optimal activity at pH 7.0 and 70°C. The enzyme retained about 90% of its activity after incubation for 30 min at 70°C. The enzyme oxidized 3β-hydroxysteroids such as cholesterol, β-cholestanol, and β-sitosterol at high rates. The Km value and Vmax value for the cholesterol were 92.6 μM and 15.9 μmol/min/mg of protein, respectively. The Vmax value of the enzyme was higher than those of commercially available cholesterol oxidases. This is the first report to characterize a cholesterol oxidase from P. aeruginosa." @default.
• W2072275500 created "2016-06-24" @default.
• W2072275500 creator A5029903489 @default.
• W2072275500 creator A5051869929 @default.
• W2072275500 date "2015-07-01" @default.
• W2072275500 modified "2023-10-18" @default.
• W2072275500 title "Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa: Screening, molecular genetic analysis, expression and characterization" @default.
• W2072275500 cites W1499139681 @default.
• W2072275500 cites W1871618408 @default.
• W2072275500 cites W1931534490 @default.
• W2072275500 cites W1969424716 @default.
• W2072275500 cites W1971385833 @default.
• W2072275500 cites W1974257218 @default.
• W2072275500 cites W1978957493 @default.
• W2072275500 cites W1989673729 @default.
• W2072275500 cites W1997627465 @default.
• W2072275500 cites W1998351717 @default.
• W2072275500 cites W2030340297 @default.
• W2072275500 cites W2032513376 @default.
• W2072275500 cites W2036724345 @default.
• W2072275500 cites W2041617853 @default.
• W2072275500 cites W2044500914 @default.
• W2072275500 cites W2052902273 @default.
• W2072275500 cites W2055043387 @default.
• W2072275500 cites W2056191407 @default.
• W2072275500 cites W2063405274 @default.
• W2072275500 cites W2072812562 @default.
• W2072275500 cites W2082935518 @default.
• W2072275500 cites W2100090449 @default.
• W2072275500 cites W2109643203 @default.
• W2072275500 cites W2117656863 @default.
• W2072275500 cites W2117783158 @default.
• W2072275500 cites W2122283423 @default.
• W2072275500 cites W2130911271 @default.
• W2072275500 cites W2135491076 @default.
• W2072275500 cites W2150361269 @default.
• W2072275500 cites W2159731899 @default.
• W2072275500 cites W2163837669 @default.
• W2072275500 cites W2171071162 @default.
• W2072275500 cites W2322520070 @default.
• W2072275500 cites W4253935212 @default.
• W2072275500 cites W4293247451 @default.
• W2072275500 doi "https://doi.org/10.1016/j.jbiosc.2014.12.003" @default.
• W2072275500 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25573142" @default.
• W2072275500 hasPublicationYear "2015" @default.
• W2072275500 type Work @default.
• W2072275500 sameAs 2072275500 @default.
• W2072275500 citedByCount "18" @default.
• W2072275500 countsByYear W20722755002017 @default.
• W2072275500 countsByYear W20722755002019 @default.
• W2072275500 countsByYear W20722755002020 @default.
• W2072275500 countsByYear W20722755002021 @default.
• W2072275500 countsByYear W20722755002022 @default.
• W2072275500 countsByYear W20722755002023 @default.
• W2072275500 crossrefType "journal-article" @default.
• W2072275500 hasAuthorship W2072275500A5029903489 @default.
• W2072275500 hasAuthorship W2072275500A5051869929 @default.
• W2072275500 hasConcept C104317684 @default.
• W2072275500 hasConcept C105702510 @default.
• W2072275500 hasConcept C153911025 @default.
• W2072275500 hasConcept C181199279 @default.
• W2072275500 hasConcept C185592680 @default.
• W2072275500 hasConcept C2776738503 @default.
• W2072275500 hasConcept C2777637488 @default.
• W2072275500 hasConcept C2778022156 @default.
• W2072275500 hasConcept C2778163477 @default.
• W2072275500 hasConcept C2779387492 @default.
• W2072275500 hasConcept C28212737 @default.
• W2072275500 hasConcept C38485361 @default.
• W2072275500 hasConcept C40767141 @default.
• W2072275500 hasConcept C523546767 @default.
• W2072275500 hasConcept C54355233 @default.
• W2072275500 hasConcept C547475151 @default.
• W2072275500 hasConcept C55493867 @default.
• W2072275500 hasConcept C86803240 @default.
• W2072275500 hasConceptScore W2072275500C104317684 @default.
• W2072275500 hasConceptScore W2072275500C105702510 @default.
• W2072275500 hasConceptScore W2072275500C153911025 @default.
• W2072275500 hasConceptScore W2072275500C181199279 @default.
• W2072275500 hasConceptScore W2072275500C185592680 @default.
• W2072275500 hasConceptScore W2072275500C2776738503 @default.
• W2072275500 hasConceptScore W2072275500C2777637488 @default.
• W2072275500 hasConceptScore W2072275500C2778022156 @default.
• W2072275500 hasConceptScore W2072275500C2778163477 @default.
• W2072275500 hasConceptScore W2072275500C2779387492 @default.
• W2072275500 hasConceptScore W2072275500C28212737 @default.
• W2072275500 hasConceptScore W2072275500C38485361 @default.
• W2072275500 hasConceptScore W2072275500C40767141 @default.
• W2072275500 hasConceptScore W2072275500C523546767 @default.
• W2072275500 hasConceptScore W2072275500C54355233 @default.
• W2072275500 hasConceptScore W2072275500C547475151 @default.
• W2072275500 hasConceptScore W2072275500C55493867 @default.
• W2072275500 hasConceptScore W2072275500C86803240 @default.
• W2072275500 hasFunder F4320320912 @default.
• W2072275500 hasFunder F4320334764 @default.
• W2072275500 hasIssue "1" @default.
• W2072275500 hasLocation W20722755001 @default.
• W2072275500 hasLocation W20722755002 @default.

• next