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- W2072291809 abstract "A technique for the rapid freezing of tissues is described, which results in the absence of icy crystals to a depth of several microns from the surface, even in large and irregular specimens. A tissue sample is instantaneously frozen by exposure to a high velocity jet of liquid propone at its freezing point. The specimen is then freeze-substituted in ether and osmium tetroxide, embedded, and sectioned for electron microscopy. Cytoplasmic fixation is excellent, and microtubules as well as other organelles and membranes are well defined." @default.
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- W2072291809 date "1978-01-01" @default.
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- W2072291809 title "Cryofixation of tissue surfaces by a propane jet for electron microscopy" @default.
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- W2072291809 doi "https://doi.org/10.1016/0047-7206(78)90020-1" @default.
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