Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072292893> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W2072292893 endingPage "61" @default.
- W2072292893 startingPage "61" @default.
- W2072292893 abstract "Abstract Transplantation in primary and secondary fetal sheep recipients was used to access the in vivo hematopoietic potential of human neuronal stem cells (NSC). Eleven primary recipients were transplanted with NSC (8×10 5 cells/fetus) and analyzed for hematopoietic chimerism at 60 days and at 6, 8 and 15 months post-transplant. At 60 days post-transplant human cells were detected in liver, muscle, spleen, thymus and bone marrow. However, at all later time points flow cytometric analysis of PB and BM revealed the presence of human multilineage hematopoietic engraftment but not CD34 + cells. PCR analysis confirmed the presence of human cells in PB and BM and further demonstrated the presence of human cells in liver, thymus, and spleen. At 6 months post-transplant primary sheep were mobilized with G-CSF (5mcg/Kg). The mobilization pattern of sheep engrafted with NSC different from those engrafted with human BM HSC. Unlike HSC sheep, in NSC animals, except for the appearance of 0.12% CD34+ cells in PB on day 3, human cell activity remained low in both BM and PB throughout mobilization. Clonogenic assay of BM cells at 8 months revealed that of the 53 hematopoietic colonies analyzed on day 19 of culture, 8 colonies were of human origin by both karyotype and PCR. At 15 months post-transplant human CD45+/HLA-DR + cells were obtained from BM of primary sheep by sorting and transplanted into secondary recipients. FACS and PCR analyses of secondary recipients demonstrated the presence of human cells inthe BM and Spleen. These results demonstrate the hematopoietic potential of human NSC and suggest that their hematopoietic activity may be regulated differently from those of human HSC in this xenogeneic model of human hematopoiesis." @default.
- W2072292893 created "2016-06-24" @default.
- W2072292893 creator A5002550316 @default.
- W2072292893 creator A5021272539 @default.
- W2072292893 creator A5034171601 @default.
- W2072292893 creator A5048464637 @default.
- W2072292893 creator A5052726807 @default.
- W2072292893 creator A5059937255 @default.
- W2072292893 creator A5091067782 @default.
- W2072292893 date "2000-07-01" @default.
- W2072292893 modified "2023-10-15" @default.
- W2072292893 title "In vivo hematopoietic potential of human neuronal stem cells" @default.
- W2072292893 doi "https://doi.org/10.1016/s0301-472x(00)00278-2" @default.
- W2072292893 hasPublicationYear "2000" @default.
- W2072292893 type Work @default.
- W2072292893 sameAs 2072292893 @default.
- W2072292893 citedByCount "4" @default.
- W2072292893 crossrefType "journal-article" @default.
- W2072292893 hasAuthorship W2072292893A5002550316 @default.
- W2072292893 hasAuthorship W2072292893A5021272539 @default.
- W2072292893 hasAuthorship W2072292893A5034171601 @default.
- W2072292893 hasAuthorship W2072292893A5048464637 @default.
- W2072292893 hasAuthorship W2072292893A5052726807 @default.
- W2072292893 hasAuthorship W2072292893A5059937255 @default.
- W2072292893 hasAuthorship W2072292893A5091067782 @default.
- W2072292893 hasBestOaLocation W20722928931 @default.
- W2072292893 hasConcept C109159458 @default.
- W2072292893 hasConcept C207001950 @default.
- W2072292893 hasConcept C28328180 @default.
- W2072292893 hasConcept C54355233 @default.
- W2072292893 hasConcept C86803240 @default.
- W2072292893 hasConcept C95444343 @default.
- W2072292893 hasConceptScore W2072292893C109159458 @default.
- W2072292893 hasConceptScore W2072292893C207001950 @default.
- W2072292893 hasConceptScore W2072292893C28328180 @default.
- W2072292893 hasConceptScore W2072292893C54355233 @default.
- W2072292893 hasConceptScore W2072292893C86803240 @default.
- W2072292893 hasConceptScore W2072292893C95444343 @default.
- W2072292893 hasIssue "7" @default.
- W2072292893 hasLocation W20722928931 @default.
- W2072292893 hasOpenAccess W2072292893 @default.
- W2072292893 hasPrimaryLocation W20722928931 @default.
- W2072292893 hasRelatedWork W1967114867 @default.
- W2072292893 hasRelatedWork W1975268103 @default.
- W2072292893 hasRelatedWork W1996916774 @default.
- W2072292893 hasRelatedWork W2033885445 @default.
- W2072292893 hasRelatedWork W2099977590 @default.
- W2072292893 hasRelatedWork W2119511363 @default.
- W2072292893 hasRelatedWork W2120223845 @default.
- W2072292893 hasRelatedWork W2121709505 @default.
- W2072292893 hasRelatedWork W3133629953 @default.
- W2072292893 hasRelatedWork W3182217228 @default.
- W2072292893 hasVolume "28" @default.
- W2072292893 isParatext "false" @default.
- W2072292893 isRetracted "false" @default.
- W2072292893 magId "2072292893" @default.
- W2072292893 workType "article" @default.