FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072332012> ?p ?o ?g. }

• W2072332012 abstract "Abstract Human granulocytes free of other cell types were obtained by counterflow centrifugation, cryogenically preserved, and studied for stability and function after thawing. Isolation of granulocytes by counterflow centrifugation was optimal at reduced temperatures (4–10 °C) in phosphate-buffered saline (or Ca2+-free buffers) at pH 7.1. A stabilizing protein, or HES was required. Routinely, 1.2% human or bovine serum albumin was used. Hyperosmolar (310 m0sm) buffers and post isolation handling in ice water baths was optimal for cryogenic preservation. Addition of DMSO at 22 °C produced transient shrinkage initially which depended on the rate of addition, concentration, and temperature. Within 10–15 min granulocytes returned to volume, but continued to swell, equilibrating for 1 hr at 20% larger volume. Ethidium uptake gradually increased. After 24 hr, extreme swelling, lysis, and ethidium uptake was observed at the highest concentration (10%) of DMSO. DMSO-induced swelling was prevented with HES. Granulocytes (30 × 106 − 50 × 106) were frozen in 2.0-ml volumes in plastic tubes. The combination of 5% DMSO, 6% HES, 4% albumin, 0.056 M glucose in NormosolR at pH 7.1 produced the best yields. Granulocytes were first cooled to 4 °C, then to −80 °C (approx rate 4 °C per min) in a mechanical freezer and finally stored in liquid nitrogen. Storage varied from days to months. Granulocytes were thawed at 42 °C by manually twirling the freezing tubes and they were subsequently maintained in ice water. They were diluted 3:1 dropwise with a room temperature solution of 7% HES, 1.2% albumin, and 0.026 M glucose in Normosol. Particle ingestion tests were conducted by incubation at room temperature for forty minutes with yeast or zymosan opsonized with autologous serum. Particles ingested were counted by microfluorimetry after two washings at 150g. Granulocytes could not be cryogenically preserved in plasma or serum. Heating or prefreezing of serum was ineffective, but dialysis or addition of EDTA overcame the destructive effect of serum. Neither treatment was an improvement over the standard freeze procedure using buffered albumin and cryoprotective components. β-mercaptoethanol added to the freezing medium caused the production of a single homogeneous population of osmotically inert, nonviable, ethidium-reactive granulocytes. This suggests that osmoregulation by granulocyte membranes is a critical requirement for cryopreservation. Preservation efficiency is species dependent, increasing in the order of human, baboon, guinea pig, and dog. Dog granulocytes can be stored for at least 8 months in liquid nitrogen with small loss of cells and functionality. The present efficiency of preservation of human granulocytes for 3–4 weeks of liquid nitrogen storage is 90–100% morphological and 40% functional recovery. Attempts to increase stability of thawed granulocytes with other additions to our current procedure have so far proved fruitless. These have consisted of inosine, adenine, pyruvate, gluconate, vitamin C, β-mercaptoethanol, para-phenylmethyl-sulfonylfluoride, and mannitol." @default.
• W2072332012 created "2016-06-24" @default.
• W2072332012 creator A5006850146 @default.
• W2072332012 creator A5049653106 @default.
• W2072332012 creator A5054190923 @default.
• W2072332012 creator A5073552203 @default.
• W2072332012 creator A5076811364 @default.
• W2072332012 date "1980-06-01" @default.
• W2072332012 modified "2023-09-25" @default.
• W2072332012 title "Factors affecting the stability of cryogenically preserved granulocytes" @default.
• W2072332012 cites W1507258866 @default.
• W2072332012 cites W1557135999 @default.
• W2072332012 cites W1860834420 @default.
• W2072332012 cites W2035389557 @default.
• W2072332012 cites W2083647986 @default.
• W2072332012 cites W2088310492 @default.
• W2072332012 cites W2090592684 @default.
• W2072332012 cites W2092819032 @default.
• W2072332012 cites W2093645124 @default.
• W2072332012 cites W2163813295 @default.
• W2072332012 cites W2409430738 @default.
• W2072332012 cites W2413163591 @default.
• W2072332012 cites W2414342425 @default.
• W2072332012 cites W2419430325 @default.
• W2072332012 cites W323658080 @default.
• W2072332012 cites W4852605 @default.
• W2072332012 doi "https://doi.org/10.1016/0011-2240(80)90037-1" @default.
• W2072332012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7408520" @default.
• W2072332012 hasPublicationYear "1980" @default.
• W2072332012 type Work @default.
• W2072332012 sameAs 2072332012 @default.
• W2072332012 citedByCount "14" @default.
• W2072332012 countsByYear W20723320122012 @default.
• W2072332012 crossrefType "journal-article" @default.
• W2072332012 hasAuthorship W2072332012A5006850146 @default.
• W2072332012 hasAuthorship W2072332012A5049653106 @default.
• W2072332012 hasAuthorship W2072332012A5054190923 @default.
• W2072332012 hasAuthorship W2072332012A5073552203 @default.
• W2072332012 hasAuthorship W2072332012A5076811364 @default.
• W2072332012 hasConcept C108939769 @default.
• W2072332012 hasConcept C121332964 @default.
• W2072332012 hasConcept C159985019 @default.
• W2072332012 hasConcept C178790620 @default.
• W2072332012 hasConcept C179933525 @default.
• W2072332012 hasConcept C185592680 @default.
• W2072332012 hasConcept C192562407 @default.
• W2072332012 hasConcept C196843134 @default.
• W2072332012 hasConcept C20556612 @default.
• W2072332012 hasConcept C2778540859 @default.
• W2072332012 hasConcept C2778769901 @default.
• W2072332012 hasConcept C43617362 @default.
• W2072332012 hasConcept C55493867 @default.
• W2072332012 hasConcept C57409179 @default.
• W2072332012 hasConcept C62520636 @default.
• W2072332012 hasConcept C7623868 @default.
• W2072332012 hasConcept C86803240 @default.
• W2072332012 hasConcept C86807702 @default.
• W2072332012 hasConcept C95444343 @default.
• W2072332012 hasConceptScore W2072332012C108939769 @default.
• W2072332012 hasConceptScore W2072332012C121332964 @default.
• W2072332012 hasConceptScore W2072332012C159985019 @default.
• W2072332012 hasConceptScore W2072332012C178790620 @default.
• W2072332012 hasConceptScore W2072332012C179933525 @default.
• W2072332012 hasConceptScore W2072332012C185592680 @default.
• W2072332012 hasConceptScore W2072332012C192562407 @default.
• W2072332012 hasConceptScore W2072332012C196843134 @default.
• W2072332012 hasConceptScore W2072332012C20556612 @default.
• W2072332012 hasConceptScore W2072332012C2778540859 @default.
• W2072332012 hasConceptScore W2072332012C2778769901 @default.
• W2072332012 hasConceptScore W2072332012C43617362 @default.
• W2072332012 hasConceptScore W2072332012C55493867 @default.
• W2072332012 hasConceptScore W2072332012C57409179 @default.
• W2072332012 hasConceptScore W2072332012C62520636 @default.
• W2072332012 hasConceptScore W2072332012C7623868 @default.
• W2072332012 hasConceptScore W2072332012C86803240 @default.
• W2072332012 hasConceptScore W2072332012C86807702 @default.
• W2072332012 hasConceptScore W2072332012C95444343 @default.
• W2072332012 hasLocation W20723320121 @default.
• W2072332012 hasLocation W20723320122 @default.
• W2072332012 hasOpenAccess W2072332012 @default.
• W2072332012 hasPrimaryLocation W20723320121 @default.
• W2072332012 hasRelatedWork W1994711691 @default.
• W2072332012 hasRelatedWork W2011881892 @default.
• W2072332012 hasRelatedWork W2162625483 @default.
• W2072332012 hasRelatedWork W2346048935 @default.
• W2072332012 hasRelatedWork W2380463747 @default.
• W2072332012 hasRelatedWork W2748952813 @default.
• W2072332012 hasRelatedWork W2899084033 @default.
• W2072332012 hasRelatedWork W2950036879 @default.
• W2072332012 hasRelatedWork W3136415254 @default.
• W2072332012 hasRelatedWork W4223546633 @default.
• W2072332012 isParatext "false" @default.
• W2072332012 isRetracted "false" @default.
• W2072332012 magId "2072332012" @default.
• W2072332012 workType "article" @default.