FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072355330> ?p ?o ?g. }

• W2072355330 endingPage "e0123379" @default.
• W2072355330 startingPage "e0123379" @default.
• W2072355330 abstract "Deamidation, the loss of the ammonium group of asparagine and glutamine to form aspartic and glutamic acid, is one of the most commonly occurring post-translational modifications in proteins. Since deamidation rates are encoded in the protein structure, it has been proposed that they can serve as molecular clocks for the timing of biological processes such as protein turnover, development and aging. Despite the importance of this process, there is a lack of detailed structural information explaining the effects of deamidation on the structure of proteins. Here, we studied the effects of deamidation on human triosephosphate isomerase (HsTIM), an enzyme for which deamidation of N15 and N71 has been long recognized as the signal for terminal marking of the protein. Deamidation was mimicked by site directed mutagenesis; thus, three mutants of HsTIM (N15D, N71D and N15D/N71D) were characterized. The results show that the N71D mutant resembles, structurally and functionally, the wild type enzyme. In contrast, the N15D mutant displays all the detrimental effects related to deamidation. The N15D/N71D mutant shows only minor additional effects when compared with the N15D mutation, supporting that deamidation of N71 induces negligible effects. The crystal structures show that, in contrast to the N71D mutant, where minimal alterations are observed, the N15D mutation forms new interactions that perturb the structure of loop 1 and loop 3, both critical components of the catalytic site and the interface of HsTIM. Based on a phylogenetic analysis of TIM sequences, we propose the conservation of this mechanism for mammalian TIMs." @default.
• W2072355330 created "2016-06-24" @default.
• W2072355330 creator A5018837516 @default.
• W2072355330 creator A5039624943 @default.
• W2072355330 creator A5047167859 @default.
• W2072355330 creator A5051688631 @default.
• W2072355330 creator A5057723387 @default.
• W2072355330 creator A5067012466 @default.
• W2072355330 creator A5067117517 @default.
• W2072355330 creator A5073784761 @default.
• W2072355330 creator A5078382501 @default.
• W2072355330 creator A5078627372 @default.
• W2072355330 creator A5080329735 @default.
• W2072355330 creator A5089881490 @default.
• W2072355330 date "2015-04-17" @default.
• W2072355330 modified "2023-10-16" @default.
• W2072355330 title "Structural Effects of Protein Aging: Terminal Marking by Deamidation in Human Triosephosphate Isomerase" @default.
• W2072355330 cites W1926915036 @default.
• W2072355330 cites W1966239340 @default.
• W2072355330 cites W1969802703 @default.
• W2072355330 cites W1974311074 @default.
• W2072355330 cites W1974579447 @default.
• W2072355330 cites W1975085160 @default.
• W2072355330 cites W1978667203 @default.
• W2072355330 cites W1999047662 @default.
• W2072355330 cites W2001641653 @default.
• W2072355330 cites W2002370234 @default.
• W2072355330 cites W2009610308 @default.
• W2072355330 cites W2011533056 @default.
• W2072355330 cites W2012385113 @default.
• W2072355330 cites W2015076423 @default.
• W2072355330 cites W2024187183 @default.
• W2072355330 cites W2030284680 @default.
• W2072355330 cites W2031965983 @default.
• W2072355330 cites W2042160423 @default.
• W2072355330 cites W2046298152 @default.
• W2072355330 cites W2046520891 @default.
• W2072355330 cites W2061273150 @default.
• W2072355330 cites W2069652971 @default.
• W2072355330 cites W2070214907 @default.
• W2072355330 cites W2077765366 @default.
• W2072355330 cites W2078969200 @default.
• W2072355330 cites W2080602313 @default.
• W2072355330 cites W2085241007 @default.
• W2072355330 cites W2086020534 @default.
• W2072355330 cites W2091639850 @default.
• W2072355330 cites W2097013015 @default.
• W2072355330 cites W2097382368 @default.
• W2072355330 cites W2116395115 @default.
• W2072355330 cites W2122344923 @default.
• W2072355330 cites W2124967403 @default.
• W2072355330 cites W2131154722 @default.
• W2072355330 cites W2132632499 @default.
• W2072355330 cites W2136789143 @default.
• W2072355330 cites W2142094348 @default.
• W2072355330 cites W2144081223 @default.
• W2072355330 cites W2149040944 @default.
• W2072355330 cites W2159211495 @default.
• W2072355330 cites W2163341755 @default.
• W2072355330 cites W2322272345 @default.
• W2072355330 cites W2396688355 @default.
• W2072355330 cites W2577842378 @default.
• W2072355330 cites W2578242640 @default.
• W2072355330 cites W31465123 @default.
• W2072355330 cites W4248872320 @default.
• W2072355330 cites W99419862 @default.
• W2072355330 doi "https://doi.org/10.1371/journal.pone.0123379" @default.
• W2072355330 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4401446" @default.
• W2072355330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25884638" @default.
• W2072355330 hasPublicationYear "2015" @default.
• W2072355330 type Work @default.
• W2072355330 sameAs 2072355330 @default.
• W2072355330 citedByCount "18" @default.
• W2072355330 countsByYear W20723553302016 @default.
• W2072355330 countsByYear W20723553302017 @default.
• W2072355330 countsByYear W20723553302018 @default.
• W2072355330 countsByYear W20723553302019 @default.
• W2072355330 countsByYear W20723553302020 @default.
• W2072355330 countsByYear W20723553302021 @default.
• W2072355330 countsByYear W20723553302022 @default.
• W2072355330 countsByYear W20723553302023 @default.
• W2072355330 crossrefType "journal-article" @default.
• W2072355330 hasAuthorship W2072355330A5018837516 @default.
• W2072355330 hasAuthorship W2072355330A5039624943 @default.
• W2072355330 hasAuthorship W2072355330A5047167859 @default.
• W2072355330 hasAuthorship W2072355330A5051688631 @default.
• W2072355330 hasAuthorship W2072355330A5057723387 @default.
• W2072355330 hasAuthorship W2072355330A5067012466 @default.
• W2072355330 hasAuthorship W2072355330A5067117517 @default.
• W2072355330 hasAuthorship W2072355330A5073784761 @default.
• W2072355330 hasAuthorship W2072355330A5078382501 @default.
• W2072355330 hasAuthorship W2072355330A5078627372 @default.
• W2072355330 hasAuthorship W2072355330A5080329735 @default.
• W2072355330 hasAuthorship W2072355330A5089881490 @default.
• W2072355330 hasBestOaLocation W20723553301 @default.
• W2072355330 hasConcept C104317684 @default.
• W2072355330 hasConcept C143065580 @default.
• W2072355330 hasConcept C16318435 @default.

• next