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- W2072363430 abstract "The combination of an enzyme-based biosensor and alkaline hydrolysis was developed for the measurement of poly(3-hydroxybutyrate) (PHB). The principle of the determination is based on that the alkaline condition converts PHB to produce its monomer, 3-hydroxybutyrate (3-HB), which generates a detectable current signal by an amperometric biosensor through coupled two-enzyme reactions on an electrode. This method takes less than 40 min, and results in a linear detection range of 0.5–110 mg L–1 PHB with a detection limit of 0.3 mg L–1 by the saturated production of 3-HB; it can also take less than 15 min and result in a linear detection range of 1.0–160 mg L–1 PHB with a detection limit of 0.5 mg L–1 by a part production of 3-HB. The method also shows simple operation and high reproducibility." @default.
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- W2072363430 date "2006-10-01" @default.
- W2072363430 modified "2023-09-30" @default.
- W2072363430 title "Determination of Poly(3-hydroxybutyrate) Using a Combination of Enzyme-based Biosensor and Alkaline Hydrolysis" @default.
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- W2072363430 doi "https://doi.org/10.2116/analsci.22.1323" @default.
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