FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072371310> ?p ?o ?g. }

• W2072371310 endingPage "48" @default.
• W2072371310 startingPage "41" @default.
• W2072371310 abstract "We have used purified RSF1010 mobilization proteins to reproduce in vitro a strand-specific nicking at the plasmid origin of transfer, oriT . On the presence of Mg 2+ , the proteins MobA (78-kDa form of RSF1010 DMA primase), MobB, and MobC and supercoiled or linear duplex oriT DNA form large amounts of a cleavage complex, which is characterized by its sensitivity to protein-denaturant treatment. Upon addition of SOS to such a complex, a single strand break is generated in the DNA, and MobA is found linked to the 5' nick terminus, presumably covalently. The double-strand nicking activity of MobA requires, in addition to Mg 2+ , the presence of MobC and is stimulated by the presence of MobB. The nick site has been shown by DNA sequencing to lie at the position cleaved in vivo during transfer, between nucleotides 3138/3139 in the r strand of RSF1010. We have found that MobA will also cleave DNA at sites other than oriT if the DNA is present in single-stranded form. Breakage in this case occurs in the absence of denaturing conditions, and after prolonged incubation, reclosure can be demonstrated." @default.
• W2072371310 created "2016-06-24" @default.
• W2072371310 creator A5036429483 @default.
• W2072371310 creator A5043981213 @default.
• W2072371310 creator A5078683049 @default.
• W2072371310 creator A5082517458 @default.
• W2072371310 date "1992-01-01" @default.
• W2072371310 modified "2023-10-16" @default.
• W2072371310 title "<i>In vitro</i>cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins" @default.
• W2072371310 cites W1516988149 @default.
• W2072371310 cites W1558516755 @default.
• W2072371310 cites W1561659773 @default.
• W2072371310 cites W1573203745 @default.
• W2072371310 cites W1579921798 @default.
• W2072371310 cites W1584533406 @default.
• W2072371310 cites W1589278627 @default.
• W2072371310 cites W1601687245 @default.
• W2072371310 cites W1859963468 @default.
• W2072371310 cites W1974581010 @default.
• W2072371310 cites W2013200026 @default.
• W2072371310 cites W2015280465 @default.
• W2072371310 cites W2022260883 @default.
• W2072371310 cites W2028240281 @default.
• W2072371310 cites W2030854358 @default.
• W2072371310 cites W2044763769 @default.
• W2072371310 cites W2047043554 @default.
• W2072371310 cites W2049147540 @default.
• W2072371310 cites W2078894194 @default.
• W2072371310 cites W2090859004 @default.
• W2072371310 cites W2132417094 @default.
• W2072371310 cites W2149721364 @default.
• W2072371310 cites W2155684957 @default.
• W2072371310 cites W322703738 @default.
• W2072371310 doi "https://doi.org/10.1093/nar/20.1.41" @default.
• W2072371310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/310323" @default.
• W2072371310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1738602" @default.
• W2072371310 hasPublicationYear "1992" @default.
• W2072371310 type Work @default.
• W2072371310 sameAs 2072371310 @default.
• W2072371310 citedByCount "92" @default.
• W2072371310 countsByYear W20723713102012 @default.
• W2072371310 countsByYear W20723713102013 @default.
• W2072371310 countsByYear W20723713102015 @default.
• W2072371310 countsByYear W20723713102016 @default.
• W2072371310 countsByYear W20723713102017 @default.
• W2072371310 countsByYear W20723713102020 @default.
• W2072371310 countsByYear W20723713102023 @default.
• W2072371310 crossrefType "journal-article" @default.
• W2072371310 hasAuthorship W2072371310A5036429483 @default.
• W2072371310 hasAuthorship W2072371310A5043981213 @default.
• W2072371310 hasAuthorship W2072371310A5078683049 @default.
• W2072371310 hasAuthorship W2072371310A5082517458 @default.
• W2072371310 hasBestOaLocation W20723713102 @default.
• W2072371310 hasConcept C104317684 @default.
• W2072371310 hasConcept C12554922 @default.
• W2072371310 hasConcept C151730666 @default.
• W2072371310 hasConcept C153911025 @default.
• W2072371310 hasConcept C156719811 @default.
• W2072371310 hasConcept C158456008 @default.
• W2072371310 hasConcept C175156509 @default.
• W2072371310 hasConcept C185581394 @default.
• W2072371310 hasConcept C194830204 @default.
• W2072371310 hasConcept C195116880 @default.
• W2072371310 hasConcept C22744801 @default.
• W2072371310 hasConcept C31254050 @default.
• W2072371310 hasConcept C43369102 @default.
• W2072371310 hasConcept C512185932 @default.
• W2072371310 hasConcept C552990157 @default.
• W2072371310 hasConcept C55493867 @default.
• W2072371310 hasConcept C67705224 @default.
• W2072371310 hasConcept C73573662 @default.
• W2072371310 hasConcept C86803240 @default.
• W2072371310 hasConceptScore W2072371310C104317684 @default.
• W2072371310 hasConceptScore W2072371310C12554922 @default.
• W2072371310 hasConceptScore W2072371310C151730666 @default.
• W2072371310 hasConceptScore W2072371310C153911025 @default.
• W2072371310 hasConceptScore W2072371310C156719811 @default.
• W2072371310 hasConceptScore W2072371310C158456008 @default.
• W2072371310 hasConceptScore W2072371310C175156509 @default.
• W2072371310 hasConceptScore W2072371310C185581394 @default.
• W2072371310 hasConceptScore W2072371310C194830204 @default.
• W2072371310 hasConceptScore W2072371310C195116880 @default.
• W2072371310 hasConceptScore W2072371310C22744801 @default.
• W2072371310 hasConceptScore W2072371310C31254050 @default.
• W2072371310 hasConceptScore W2072371310C43369102 @default.
• W2072371310 hasConceptScore W2072371310C512185932 @default.
• W2072371310 hasConceptScore W2072371310C552990157 @default.
• W2072371310 hasConceptScore W2072371310C55493867 @default.
• W2072371310 hasConceptScore W2072371310C67705224 @default.
• W2072371310 hasConceptScore W2072371310C73573662 @default.
• W2072371310 hasConceptScore W2072371310C86803240 @default.
• W2072371310 hasIssue "1" @default.
• W2072371310 hasLocation W20723713101 @default.
• W2072371310 hasLocation W20723713102 @default.
• W2072371310 hasLocation W20723713103 @default.
• W2072371310 hasLocation W20723713104 @default.
• W2072371310 hasOpenAccess W2072371310 @default.
• W2072371310 hasPrimaryLocation W20723713101 @default.

• next