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- W2072371349 abstract "Hepatocytes are highly polarized cells where intercellular junctions, including tight junctions (TJs), determine the polarity. Recently, the TJ-associated proteins claudin-1 and occludin have been implicated in hepatitis C virus (HCV) entry and spread. Nevertheless, cell line-based experimental systems that exhibit hepatocyte-like polarity and permit robust infection and virion production are not currently available. Thus, we sought to determine whether cell line-based, Matrigel-embedded cultures could be used to study hepatitis C virus (HCV) infection and virion production in a context of hepatocyte-like polarized cells. In contrast to standard bidimensional cultures, Matrigel-cultured Huh-7 cells adopted hepatocyte polarization features forming a continuous network of functional proto-bile canaliculi structures. These 3D cultures supported HCV infection by JFH-1 virus and produced infective viral particles which shifted towards lower densities with higher associated specific infectivity. In conclusion, our findings describe a novel use of Matrigel to study the entire HCV cycle in a more relevant context." @default.
- W2072371349 created "2016-06-24" @default.
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- W2072371349 date "2012-03-01" @default.
- W2072371349 modified "2023-10-18" @default.
- W2072371349 title "Matrigel-embedded 3D culture of Huh-7 cells as a hepatocyte-like polarized system to study hepatitis C virus cycle" @default.
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- W2072371349 doi "https://doi.org/10.1016/j.virol.2011.12.021" @default.
- W2072371349 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22280897" @default.
- W2072371349 hasPublicationYear "2012" @default.
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