Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072394174> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2072394174 endingPage "3698" @default.
- W2072394174 startingPage "3690" @default.
- W2072394174 abstract "Copper amine oxidases possess the unusual ability to generate autocatalytically their organic cofactor, which is subsequently utilized in turnover. This cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ), is formed within the active site of these enzymes by the oxidation of a single tyrosine residue. In vitro, copper(II) and oxygen are both necessary and sufficient for the conversion of tyrosine to TPQ. In this study, the biogenesis of TPQ has been characterized in an amine oxidase from Hansenula polymorpha expressed as the apo-enzyme in Escherichia coli. With the WT enzyme, optical absorbances which are copper or oxygen dependent are observed and characterized. Active-site mutants are used to investigate further the nature of these spectral species. Evidence is presented which suggests that tyrosine is activated for reaction with oxygen by liganding to Cu(II). In the following paper in this issue [Schwartz, B., Dove, J. E., and Klinman, J. P. (2000) Biochemistry 39, 3699-3707], the initial reaction of precursor protein with oxygen is characterized kinetically. Taken together, the available data suggest a mechanism for the oxidation of tyrosine to TPQ where the role of the copper is to activate substrate." @default.
- W2072394174 created "2016-06-24" @default.
- W2072394174 creator A5025626775 @default.
- W2072394174 creator A5041988653 @default.
- W2072394174 creator A5058352806 @default.
- W2072394174 creator A5066409965 @default.
- W2072394174 date "2000-03-10" @default.
- W2072394174 modified "2023-10-02" @default.
- W2072394174 title "Investigation of Spectroscopic Intermediates during Copper-Binding and TPQ Formation in Wild-Type and Active-Site Mutants of a Copper-Containing Amine Oxidase from Yeast" @default.
- W2072394174 cites W1981940760 @default.
- W2072394174 cites W1983672252 @default.
- W2072394174 cites W1996107817 @default.
- W2072394174 cites W2005575697 @default.
- W2072394174 cites W2023756858 @default.
- W2072394174 cites W2063081233 @default.
- W2072394174 cites W2076420253 @default.
- W2072394174 cites W2116838198 @default.
- W2072394174 cites W2174630077 @default.
- W2072394174 doi "https://doi.org/10.1021/bi992225w" @default.
- W2072394174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10736168" @default.
- W2072394174 hasPublicationYear "2000" @default.
- W2072394174 type Work @default.
- W2072394174 sameAs 2072394174 @default.
- W2072394174 citedByCount "61" @default.
- W2072394174 countsByYear W20723941742012 @default.
- W2072394174 countsByYear W20723941742013 @default.
- W2072394174 countsByYear W20723941742014 @default.
- W2072394174 countsByYear W20723941742016 @default.
- W2072394174 countsByYear W20723941742017 @default.
- W2072394174 countsByYear W20723941742018 @default.
- W2072394174 countsByYear W20723941742019 @default.
- W2072394174 countsByYear W20723941742021 @default.
- W2072394174 countsByYear W20723941742022 @default.
- W2072394174 countsByYear W20723941742023 @default.
- W2072394174 crossrefType "journal-article" @default.
- W2072394174 hasAuthorship W2072394174A5025626775 @default.
- W2072394174 hasAuthorship W2072394174A5041988653 @default.
- W2072394174 hasAuthorship W2072394174A5058352806 @default.
- W2072394174 hasAuthorship W2072394174A5066409965 @default.
- W2072394174 hasConcept C117001961 @default.
- W2072394174 hasConcept C131779359 @default.
- W2072394174 hasConcept C178790620 @default.
- W2072394174 hasConcept C181199279 @default.
- W2072394174 hasConcept C185592680 @default.
- W2072394174 hasConcept C197957613 @default.
- W2072394174 hasConcept C2776165026 @default.
- W2072394174 hasConcept C2779053737 @default.
- W2072394174 hasConcept C2779183435 @default.
- W2072394174 hasConcept C2780643102 @default.
- W2072394174 hasConcept C38485361 @default.
- W2072394174 hasConcept C41183919 @default.
- W2072394174 hasConcept C544778455 @default.
- W2072394174 hasConcept C55493867 @default.
- W2072394174 hasConcept C71240020 @default.
- W2072394174 hasConceptScore W2072394174C117001961 @default.
- W2072394174 hasConceptScore W2072394174C131779359 @default.
- W2072394174 hasConceptScore W2072394174C178790620 @default.
- W2072394174 hasConceptScore W2072394174C181199279 @default.
- W2072394174 hasConceptScore W2072394174C185592680 @default.
- W2072394174 hasConceptScore W2072394174C197957613 @default.
- W2072394174 hasConceptScore W2072394174C2776165026 @default.
- W2072394174 hasConceptScore W2072394174C2779053737 @default.
- W2072394174 hasConceptScore W2072394174C2779183435 @default.
- W2072394174 hasConceptScore W2072394174C2780643102 @default.
- W2072394174 hasConceptScore W2072394174C38485361 @default.
- W2072394174 hasConceptScore W2072394174C41183919 @default.
- W2072394174 hasConceptScore W2072394174C544778455 @default.
- W2072394174 hasConceptScore W2072394174C55493867 @default.
- W2072394174 hasConceptScore W2072394174C71240020 @default.
- W2072394174 hasIssue "13" @default.
- W2072394174 hasLocation W20723941741 @default.
- W2072394174 hasLocation W20723941742 @default.
- W2072394174 hasOpenAccess W2072394174 @default.
- W2072394174 hasPrimaryLocation W20723941741 @default.
- W2072394174 hasRelatedWork W2014556749 @default.
- W2072394174 hasRelatedWork W2015170546 @default.
- W2072394174 hasRelatedWork W2049934076 @default.
- W2072394174 hasRelatedWork W2055655058 @default.
- W2072394174 hasRelatedWork W2059466750 @default.
- W2072394174 hasRelatedWork W2072394174 @default.
- W2072394174 hasRelatedWork W2077211817 @default.
- W2072394174 hasRelatedWork W217893489 @default.
- W2072394174 hasRelatedWork W2240264931 @default.
- W2072394174 hasRelatedWork W2963179619 @default.
- W2072394174 hasVolume "39" @default.
- W2072394174 isParatext "false" @default.
- W2072394174 isRetracted "false" @default.
- W2072394174 magId "2072394174" @default.
- W2072394174 workType "article" @default.