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- W2072404714 abstract "The aim of this study was to investigate the effect of static magnetic field (SMF) exposure in antioxidant enzyme activity, the labile zinc fraction and DNA damage in THP1 cells (monocyte line). Cell culture flasks were exposed to SMF (250 mT) during 1 h (group 1), 2 h (group 2) and 3 h (group 3). Our results showed that cell viability was slightly lower in SMF-exposed groups compared to a sham exposed group. However, SMF exposure failed to alter malondialdehyde (MDA) concentration (+6%, p > 0.05) and glutathione peroxidase (GPx) (−5%, p > 0.05), catalase (CAT) (−6%, p > 0.05) and superoxide dismutase (SOD) activities (+38%, p > 0.05) in group 3 compared to the sham exposed group. DNA analysis by single cell gel electrophoresis (comet assay) revealed that SMF exposure did not exert any DNA damage in groups 1 and 2. However, it induced a low level of DNA single strand breaks in cells of group 3. To further explore the oxidative DNA damage, cellular DNA for group 3 was isolated, hydrolyzed and analysed by HPLC-EC. The level of 8-oxodGuo in this group remained unchanged compared to the sham exposed group (+6.5%, p > 0.05). Cells stained with zinc-specific fluorescent probes zinpyr-1 showed a decrease of labile zinc fraction in all groups exposed to SMF." @default.
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- W2072404714 date "2007-01-22" @default.
- W2072404714 modified "2023-10-18" @default.
- W2072404714 title "Influence of a static magnetic field (250 mT) on the antioxidant response and DNA integrity in THP1 cells" @default.
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- W2072404714 doi "https://doi.org/10.1088/0031-9155/52/4/002" @default.
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