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- W2072423511 abstract "We attempted to expand the range of chlorinated solvents degraded by Xanthobacter autotrophicus GJ10 to include trichloroethylene by the rational modification of the enzyme haloalkane dehalogenase. The amino acids Phe164, Asp170, Phe172 and Trp175 were individually replaced with alanine by site-directed mutagenesis. All substitutions produced enzymes with lower than wild type activity with 1,2-dichloroethane. The Phe164Ala and Asp170Ala mutants were 3 and 2 times more active than was the wild type enzyme in dechlorinating 1,6-dichlorohexane. The Asp170Ala mutant resembled the wild type enzyme in its relative activity against longer chain substrates. No mutant was active with trichloroethylene. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 520–523, 1998." @default.
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- W2072423511 date "1998-08-20" @default.
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- W2072423511 title "Alteration of the substrate range of haloalkane dehalogenase by site-directed mutagenesis" @default.
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- W2072423511 doi "https://doi.org/10.1002/(sici)1097-0290(19980820)59:4<520::aid-bit16>3.0.co;2-d" @default.
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