FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072430487> ?p ?o ?g. }

• W2072430487 endingPage "1199" @default.
• W2072430487 startingPage "1187" @default.
• W2072430487 abstract "We propose a mild stepwise fractionation of molecular components of a humic acid (HA) suprastructure and their structural identification by advanced analytical methods. This procedure may be the basis of a Humeomics approach to characterize natural humic molecules and clarify their relations with ecosystems functions. Sequential fractionation included: (1) organic solvent extraction, (2) transesterification with boron trifluoride in methanol (BF(3)-CH(3)OH), (3) methanolic alkaline hydrolysis (KOH-CH(3)OH), and (4) cleavage of ether and glycosidic bonds with HI. Structural identification of initial and final material, separated organo-soluble and hydrosoluble fractions, and subfractions was conducted by GC-MS, HPSEC-ESI-MS (high-resolution, Orbitrap), and solid- and liquid-state NMR. GC-MS revealed in organosoluble unbound fractions the presence of both saturated and unsaturated, linear and branched, alkanoic, hydroxyalkanoic and alkandioic acids, n-alkanes, and n-alkanols. These components decreased progressively in fractions obtained after weak and strong ester cleavage. Unsubstituted alkanoic acids with variable chain length were ubiquitously detected in all fractions, thereby suggesting their fundamental function in the architecture of humic suprastructures. An important role in differentiating supramolecular associations should also be attributed to substituted alkanoic acids that were detected in variable amounts in different fractions. The content of aromatic acids and steroids was only noticed in the latter fractions. HPSEC-ESI-MS of initial and final solid fractions showed similar compounds, as indicated by GC-MS, whereas the hydrosoluble fraction after transesterification revealed fewer of these compounds but noticeable nitrogen-containing acids. A large amount of cyclic acids were identified by MS empirical formula in initial HA, and, to a lesser extent, in the final fractionation residue as well as in the hydrosoluble fraction. The predominant alkyl NMR signals in organosoluble extracts and those of CH-N, CH-O, and O-CH-O groups in hydrosoluble fraction confirmed mass spectrometry results. Homo- and heterocorrelated liquid-state NMR spectra indicated spin systems interactions varying with separated fractions. Solid-state and dipolar-dephasing NMR spectra of final residue showed predominance of sp(2) carbons, 66% of which were quaternary carbons, and a significant increase in conformational rigidity with respect to initial HA. Separated fractions accounted for 60% of initial HA weight, and losses were attributed to hydration water, liberated volatile compounds, and decarboxylation. Quantization of analytes showed that the sum of compound classes in separated fractions was greater than that for the initial HA, thereby showing that stepwise fractionation increased significantly the analytical identification of humic molecules. Our results suggest this Humeomics approach as a valid path for mapping humic molecular composition and assess humus origin and formation." @default.
• W2072430487 created "2016-06-24" @default.
• W2072430487 creator A5057471395 @default.
• W2072430487 creator A5066790773 @default.
• W2072430487 date "2011-03-01" @default.
• W2072430487 modified "2023-10-10" @default.
• W2072430487 title "Basis of a Humeomics Science: Chemical Fractionation and Molecular Characterization of Humic Biosuprastructures" @default.
• W2072430487 cites W1566631867 @default.
• W2072430487 cites W1963486647 @default.
• W2072430487 cites W1964358240 @default.
• W2072430487 cites W1966522090 @default.
• W2072430487 cites W1967677705 @default.
• W2072430487 cites W1969543668 @default.
• W2072430487 cites W1971442772 @default.
• W2072430487 cites W1971983473 @default.
• W2072430487 cites W1972834641 @default.
• W2072430487 cites W1973874914 @default.
• W2072430487 cites W1974335021 @default.
• W2072430487 cites W1977420870 @default.
• W2072430487 cites W1979458831 @default.
• W2072430487 cites W1983820737 @default.
• W2072430487 cites W1986332055 @default.
• W2072430487 cites W1987093278 @default.
• W2072430487 cites W1987156875 @default.
• W2072430487 cites W1988930091 @default.
• W2072430487 cites W1992052664 @default.
• W2072430487 cites W2004714782 @default.
• W2072430487 cites W2009537509 @default.
• W2072430487 cites W2013173749 @default.
• W2072430487 cites W2019544520 @default.
• W2072430487 cites W2025528184 @default.
• W2072430487 cites W2049348853 @default.
• W2072430487 cites W2051003719 @default.
• W2072430487 cites W2058750963 @default.
• W2072430487 cites W2060926947 @default.
• W2072430487 cites W2062504058 @default.
• W2072430487 cites W2065698268 @default.
• W2072430487 cites W2073067063 @default.
• W2072430487 cites W2074305783 @default.
• W2072430487 cites W2078388104 @default.
• W2072430487 cites W2085372668 @default.
• W2072430487 cites W2089773144 @default.
• W2072430487 cites W2105601778 @default.
• W2072430487 cites W2118606727 @default.
• W2072430487 cites W2128080907 @default.
• W2072430487 cites W2129477967 @default.
• W2072430487 cites W2137216588 @default.
• W2072430487 cites W2143980952 @default.
• W2072430487 cites W2147443546 @default.
• W2072430487 cites W2150033665 @default.
• W2072430487 cites W2314373772 @default.
• W2072430487 cites W2489380103 @default.
• W2072430487 cites W4254209824 @default.
• W2072430487 cites W628036256 @default.
• W2072430487 doi "https://doi.org/10.1021/bm101488e" @default.
• W2072430487 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21361272" @default.
• W2072430487 hasPublicationYear "2011" @default.
• W2072430487 type Work @default.
• W2072430487 sameAs 2072430487 @default.
• W2072430487 citedByCount "132" @default.
• W2072430487 countsByYear W20724304872012 @default.
• W2072430487 countsByYear W20724304872013 @default.
• W2072430487 countsByYear W20724304872014 @default.
• W2072430487 countsByYear W20724304872015 @default.
• W2072430487 countsByYear W20724304872016 @default.
• W2072430487 countsByYear W20724304872017 @default.
• W2072430487 countsByYear W20724304872018 @default.
• W2072430487 countsByYear W20724304872019 @default.
• W2072430487 countsByYear W20724304872020 @default.
• W2072430487 countsByYear W20724304872021 @default.
• W2072430487 countsByYear W20724304872022 @default.
• W2072430487 countsByYear W20724304872023 @default.
• W2072430487 crossrefType "journal-article" @default.
• W2072430487 hasAuthorship W2072430487A5057471395 @default.
• W2072430487 hasAuthorship W2072430487A5066790773 @default.
• W2072430487 hasConcept C161790260 @default.
• W2072430487 hasConcept C178790620 @default.
• W2072430487 hasConcept C185592680 @default.
• W2072430487 hasConcept C2776468994 @default.
• W2072430487 hasConcept C43617362 @default.
• W2072430487 hasConcept C94412978 @default.
• W2072430487 hasConcept C97428945 @default.
• W2072430487 hasConceptScore W2072430487C161790260 @default.
• W2072430487 hasConceptScore W2072430487C178790620 @default.
• W2072430487 hasConceptScore W2072430487C185592680 @default.
• W2072430487 hasConceptScore W2072430487C2776468994 @default.
• W2072430487 hasConceptScore W2072430487C43617362 @default.
• W2072430487 hasConceptScore W2072430487C94412978 @default.
• W2072430487 hasConceptScore W2072430487C97428945 @default.
• W2072430487 hasIssue "4" @default.
• W2072430487 hasLocation W20724304871 @default.
• W2072430487 hasLocation W20724304872 @default.
• W2072430487 hasOpenAccess W2072430487 @default.
• W2072430487 hasPrimaryLocation W20724304871 @default.
• W2072430487 hasRelatedWork W1970144168 @default.
• W2072430487 hasRelatedWork W2000238513 @default.
• W2072430487 hasRelatedWork W2039637454 @default.
• W2072430487 hasRelatedWork W2063678345 @default.

• next