FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072485174> ?p ?o ?g. }

• W2072485174 endingPage "969" @default.
• W2072485174 startingPage "955" @default.
• W2072485174 abstract "The effect of His-heme misligation on folding has been investigated for a triple mutant of yeast iso-2 cytochrome c (N26H,H33N,H39K iso-2). The variant contains a single misligating His residue at position 26, a location at which His residues are found in several cytochrome c homologues, including horse, tuna, and yeast iso-1. The amplitude for fast phase folding exhibits a strong initial pH dependence. For GdnHCl unfolded protein at an initial pH<5, the observed refolding at final pH 6 is dominated by a fast phase (tau(2f)=20 ms, alpha(2f)=90 %) that represents folding in the absence of misligation. For unfolded protein at initial pH 6, folding at final pH 6 occurs in a fast phase of reduced amplitude (alpha(2f) approximately 20 %) but the same rate (tau(2f)=20 ms), and in two slower phases (tau(m)=6-8 seconds, alpha(m) approximately 45 %; and tau(1b)=16-20 seconds, alpha(1b) approximately 35 %). Double jump experiments show that the initial pH dependence of the folding amplitudes results from a slow pH-dependent equilibrium between fast and slow folding species present in the unfolded protein. The slow equilibrium arises from coupling of the His protonation equilibrium to His-heme misligation and proline isomerization. Specifically, Pro25 is predominantly in trans in the unligated low-pH unfolded protein, but is constrained in a non-native cis isomerization state by His26-heme misligation near neutral pH. Refolding from the misligated unfolded form proceeds slowly due to the large energetic barrier required for proline isomerization and displacement of the misligated His26-heme ligand." @default.
• W2072485174 created "2016-06-24" @default.
• W2072485174 creator A5046029643 @default.
• W2072485174 creator A5068448613 @default.
• W2072485174 date "2000-05-01" @default.
• W2072485174 modified "2023-10-16" @default.
• W2072485174 title "Coupled kinetic traps in cytochrome c folding: His-Heme misligation and proline isomerization" @default.
• W2072485174 cites W1493551487 @default.
• W2072485174 cites W1498222524 @default.
• W2072485174 cites W1606676886 @default.
• W2072485174 cites W1607106398 @default.
• W2072485174 cites W1967305892 @default.
• W2072485174 cites W1970804960 @default.
• W2072485174 cites W1973948986 @default.
• W2072485174 cites W1981923148 @default.
• W2072485174 cites W1984770178 @default.
• W2072485174 cites W2003153505 @default.
• W2072485174 cites W2011222301 @default.
• W2072485174 cites W2013843898 @default.
• W2072485174 cites W2028231353 @default.
• W2072485174 cites W2038263678 @default.
• W2072485174 cites W2039227541 @default.
• W2072485174 cites W2046152827 @default.
• W2072485174 cites W2054869657 @default.
• W2072485174 cites W2064996945 @default.
• W2072485174 cites W2068218021 @default.
• W2072485174 cites W2068651887 @default.
• W2072485174 cites W2076604631 @default.
• W2072485174 cites W2078362254 @default.
• W2072485174 cites W2083343752 @default.
• W2072485174 cites W2088922005 @default.
• W2072485174 cites W2090395982 @default.
• W2072485174 cites W2102577272 @default.
• W2072485174 cites W2113867108 @default.
• W2072485174 cites W2116076986 @default.
• W2072485174 cites W2117747873 @default.
• W2072485174 cites W345458310 @default.
• W2072485174 doi "https://doi.org/10.1006/jmbi.2000.3700" @default.
• W2072485174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10801361" @default.
• W2072485174 hasPublicationYear "2000" @default.
• W2072485174 type Work @default.
• W2072485174 sameAs 2072485174 @default.
• W2072485174 citedByCount "32" @default.
• W2072485174 countsByYear W20724851742012 @default.
• W2072485174 countsByYear W20724851742014 @default.
• W2072485174 countsByYear W20724851742015 @default.
• W2072485174 countsByYear W20724851742018 @default.
• W2072485174 countsByYear W20724851742022 @default.
• W2072485174 crossrefType "journal-article" @default.
• W2072485174 hasAuthorship W2072485174A5046029643 @default.
• W2072485174 hasAuthorship W2072485174A5068448613 @default.
• W2072485174 hasConcept C119599485 @default.
• W2072485174 hasConcept C126661725 @default.
• W2072485174 hasConcept C127413603 @default.
• W2072485174 hasConcept C145148216 @default.
• W2072485174 hasConcept C161790260 @default.
• W2072485174 hasConcept C178790620 @default.
• W2072485174 hasConcept C181199279 @default.
• W2072485174 hasConcept C185592680 @default.
• W2072485174 hasConcept C204328495 @default.
• W2072485174 hasConcept C2776217839 @default.
• W2072485174 hasConcept C2776545253 @default.
• W2072485174 hasConcept C2780768313 @default.
• W2072485174 hasConcept C28859421 @default.
• W2072485174 hasConcept C29311851 @default.
• W2072485174 hasConcept C30095370 @default.
• W2072485174 hasConcept C55493867 @default.
• W2072485174 hasConcept C71240020 @default.
• W2072485174 hasConcept C8010536 @default.
• W2072485174 hasConceptScore W2072485174C119599485 @default.
• W2072485174 hasConceptScore W2072485174C126661725 @default.
• W2072485174 hasConceptScore W2072485174C127413603 @default.
• W2072485174 hasConceptScore W2072485174C145148216 @default.
• W2072485174 hasConceptScore W2072485174C161790260 @default.
• W2072485174 hasConceptScore W2072485174C178790620 @default.
• W2072485174 hasConceptScore W2072485174C181199279 @default.
• W2072485174 hasConceptScore W2072485174C185592680 @default.
• W2072485174 hasConceptScore W2072485174C204328495 @default.
• W2072485174 hasConceptScore W2072485174C2776217839 @default.
• W2072485174 hasConceptScore W2072485174C2776545253 @default.
• W2072485174 hasConceptScore W2072485174C2780768313 @default.
• W2072485174 hasConceptScore W2072485174C28859421 @default.
• W2072485174 hasConceptScore W2072485174C29311851 @default.
• W2072485174 hasConceptScore W2072485174C30095370 @default.
• W2072485174 hasConceptScore W2072485174C55493867 @default.
• W2072485174 hasConceptScore W2072485174C71240020 @default.
• W2072485174 hasConceptScore W2072485174C8010536 @default.
• W2072485174 hasIssue "5" @default.
• W2072485174 hasLocation W20724851741 @default.
• W2072485174 hasLocation W20724851742 @default.
• W2072485174 hasOpenAccess W2072485174 @default.
• W2072485174 hasPrimaryLocation W20724851741 @default.
• W2072485174 hasRelatedWork W1493551487 @default.
• W2072485174 hasRelatedWork W1501456700 @default.
• W2072485174 hasRelatedWork W1607106398 @default.
• W2072485174 hasRelatedWork W1981521205 @default.
• W2072485174 hasRelatedWork W2041542205 @default.
• W2072485174 hasRelatedWork W2064871278 @default.

• next