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- W2072548356 abstract "Object. The mechanism of arterial vasoconstriction caused by oxyhemoglobin production after subarachnoid hemorrhage was investigated. Methods. Using a fluorescent Ca ++ indicator (fura-2 acetoxymethyl ester), the change in the cytosolic intracellular Ca ++ concentration, [Ca ++ ] i , was measured in cultured rat vascular smooth-muscle cells exposed to oxyhemoglobin and other substances. Oxyhemoglobin induced transient elevation of smooth-muscle cell [Ca ++ ] i in either the presence or absence of ethyleneglycol-bis (β-aminoethylether)-N,N′-tetraacetic acid, indicating that Ca ++ released by oxyhemoglobin was derived from [Ca ++ ] i stores. In contrast, methemoglobin had no effect on the smooth-muscle cells. Exposure of the cells to reactive oxygen species generated by xanthine plus xanthine oxidase yielded the same results as with oxyhemoglobin, that is, transient elevation of smooth-muscle cell [Ca ++ ] i . Procaine (a Ca ++ channel blocker) failed to inhibit the oxyhemoglobin-induced elevation of [Ca ++ ] i . Ryanodine (a Ca ++ channel opener) plus oxyhemoglobin caused markedly greater elevation of [Ca ++ ] i than ryanodine alone, whereas thapsigargin (an adenosine triphosphate [ATP]-dependent Ca ++ pump inhibitor) plus oxyhemoglobin had no additional effect when compared with thapsigargin alone. The oxyhemoglobin-induced elevation of [Ca ++ ] i could be blocked by an Fe ++ chelator (ferene), but not by an Fe +++ chelator (deferoxamine mesylate). Treatment with either dithiothreitol or glutathione monoethyl ester markedly inhibited the oxyhemoglobin-induced elevation of [Ca ++ ] i . Conclusions. These results indicate that Fe ++ -catalyzed hydroxyl radicals generated from oxyhemoglobin-derived free radicals induce the elevation of [Ca ++ ] i by inhibiting the ATP-dependent Ca ++ pump rather than the Ca ++ channels in the sarcoplasmic reticulum and that thiols may prevent Ca ++ pump inactivation by inhibiting the oxidation of membrane sulfhydryl groups." @default.
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- W2072548356 date "1999-03-01" @default.
- W2072548356 modified "2023-09-23" @default.
- W2072548356 title "Glutathione monoethyl ester and inhibition of the oxyhemoglobin-induced increase in cytosolic calcium in cultured smooth-muscle cells" @default.
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- W2072548356 doi "https://doi.org/10.3171/jns.1999.90.3.0527" @default.
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