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- W2072575327 abstract "Abstract An automated microbiological assay for the quantitation of niacin is described. The assay, involving the use of Lactobacillus plantarum, was performed in culture microplates. The performance of the assay in both 48-well and 96-well culture microplates was investigated. Pipetting was carried out by a computer-controlled laboratory robot. Growth response was read by video digital-image processing (VDIP) and by ELISA reader. The effect of shaking the microplates prior to reading was also investigated. The use of a niacin-depleted culture as inoculum shows lower blanks and an increased growth rate at low concentrations of niacin. The incubation time is reduced from 48 to 24 h by using the culture of L. plantarum directly after thawing of the cryopreserved working culture. The 48-well plate assay showed better performance than the assay performed in 96-well culture plates. The coefficient of variation (CV) was improved in the 48-well plate assay when compared with the conventional microbiological assay. The relative intra-assay variation ranged from 3.7 to 8.5% in the 48-well plate assay and from 16.7 to 25.8% in the 96-well plate assay for samples of foods and feeds. The recovery of added standard in the 48-well and in the 96-well culture-plate assay was 101% ± 3% (n = 6), and 105% ± 12% (n = 6) respectively. The results of the 48-well plate assay correlated well with the results obtained by the conventional microbiological assay (r = 0.985, n = 40). The detection limit for the 48-well assay was found to be 0.5 ng niacin per well. Avoiding shaking the 48-well plates prior to reading of growth response increased the precision significantly. The technique used in this assay reduces reagent costs and time spent on pipetting and evaluating results when compared with the conventional microbiological assay performed in 5-ml tubes. The microplate assay is more compact and easier to perform. Digital image processing is introduced as a measurement of turbidity." @default.
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- W2072575327 date "1994-01-01" @default.
- W2072575327 modified "2023-09-27" @default.
- W2072575327 title "Automated microbiological assay for quantitation of niacin performed in culture microplates read by digital image processing" @default.
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- W2072575327 doi "https://doi.org/10.1016/0308-8146(94)90015-9" @default.
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