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- W2072578105 abstract "The Amyloid-β (Aβ) peptide is a major component of the amyloid plaques associated with Alzheimer's disease (AD). Recent studies suggest that the most toxic forms of Aβ are small, soluble oligomeric aggregates. Here, we report the isolation and characterization of a single-chain variable domain (scFv) antibody isolated against oligomeric Aβ using a protocol developed in our laboratory that combines phage display technology and atomic force microscopy (AFM). Starting with a randomized, single framework phage display library, after three rounds of selection against oligomeric Aβ, we identified an scFv that bound oligomeric Aβ specifically, but not monomeric or fibrillar forms. The anti-oligomeric scFv inhibits Aβ aggregation and toxicity, and reduces the toxicity of preformed oligomeric Aβ towards human neuroblastoma cells. When used to probe samples of human brain tissue, the scFv reacted with AD tissue but not a healthy control or Parkinson's disease brain samples. The anti-oligomeric Aβ scFv therefore has potential therapeutic and diagnostic applications in specifically targeting or identifying the toxic morphologies of Aβ in AD brains." @default.
- W2072578105 created "2016-06-24" @default.
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- W2072578105 date "2008-12-01" @default.
- W2072578105 modified "2023-10-17" @default.
- W2072578105 title "Anti-oligomeric Aβ Single-chain Variable Domain Antibody Blocks Aβ-induced Toxicity Against Human Neuroblastoma Cells" @default.
- W2072578105 cites W1482580692 @default.
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- W2072578105 cites W1549258100 @default.
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- W2072578105 cites W1592702941 @default.
- W2072578105 cites W1621971853 @default.
- W2072578105 cites W1653912017 @default.
- W2072578105 cites W1693283493 @default.
- W2072578105 cites W1705725530 @default.
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- W2072578105 doi "https://doi.org/10.1016/j.jmb.2008.09.068" @default.
- W2072578105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18929576" @default.
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