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- W2072578281 abstract "Abstract A 4-step method for purification of the inhibitor has been devised. Step 1 is a precipitation procedure with polyethyleneglycol. Step 2 involves flotation centrifugation in a solution of 35% NaBr. Step 3 and Step 4 consist of chromatographic molecular sievings of agarose gels (as beads) of 8% and 4%, respectively. The two last step were performed in Tris buffer (pH 7.3) with KBr to stabilise the inhibitor. The inhibitor is probably a complex. It behaves as a γM component but it is not an ordinary γM antibody. It sertainly has a high molecular weight and seems to have very little or no lipid. The activity is increased at pH below 3.5." @default.
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- W2072578281 date "1966-09-26" @default.
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- W2072578281 title "Purification and propeties of a non-specific hemagglutination inhibitor from human serum against picornaviruses" @default.
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- W2072578281 doi "https://doi.org/10.1016/0304-4165(66)90484-3" @default.
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