FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072602203> ?p ?o ?g. }

• W2072602203 endingPage "e17820" @default.
• W2072602203 startingPage "e17820" @default.
• W2072602203 abstract "C57BL/6J (B6) and DBA/2J (D2) are two of the most commonly used inbred mouse strains in neuroscience research. However, the only currently available mouse genome is based entirely on the B6 strain sequence. Subsequently, oligonucleotide microarray probes are based solely on this B6 reference sequence, making their application for gene expression profiling comparisons across mouse strains dubious due to their allelic sequence differences, including single nucleotide polymorphisms (SNPs). The emergence of next-generation sequencing (NGS) and the RNA-Seq application provides a clear alternative to oligonucleotide arrays for detecting differential gene expression without the problems inherent to hybridization-based technologies. Using RNA-Seq, an average of 22 million short sequencing reads were generated per sample for 21 samples (10 B6 and 11 D2), and these reads were aligned to the mouse reference genome, allowing 16,183 Ensembl genes to be queried in striatum for both strains. To determine differential expression, 'digital mRNA counting' is applied based on reads that map to exons. The current study compares RNA-Seq (Illumina GA IIx) with two microarray platforms (Illumina MouseRef-8 v2.0 and Affymetrix MOE 430 2.0) to detect differential striatal gene expression between the B6 and D2 inbred mouse strains. We show that by using stringent data processing requirements differential expression as determined by RNA-Seq is concordant with both the Affymetrix and Illumina platforms in more instances than it is concordant with only a single platform, and that instances of discordance with respect to direction of fold change were rare. Finally, we show that additional information is gained from RNA-Seq compared to hybridization-based techniques as RNA-Seq detects more genes than either microarray platform. The majority of genes differentially expressed in RNA-Seq were only detected as present in RNA-Seq, which is important for studies with smaller effect sizes where the sensitivity of hybridization-based techniques could bias interpretation." @default.
• W2072602203 created "2016-06-24" @default.
• W2072602203 creator A5000665006 @default.
• W2072602203 creator A5007067790 @default.
• W2072602203 creator A5010388465 @default.
• W2072602203 creator A5010485441 @default.
• W2072602203 creator A5029270987 @default.
• W2072602203 creator A5033173474 @default.
• W2072602203 creator A5061516412 @default.
• W2072602203 creator A5066373576 @default.
• W2072602203 creator A5072087302 @default.
• W2072602203 creator A5074640629 @default.
• W2072602203 date "2011-03-24" @default.
• W2072602203 modified "2023-09-26" @default.
• W2072602203 title "Evaluating Gene Expression in C57BL/6J and DBA/2J Mouse Striatum Using RNA-Seq and Microarrays" @default.
• W2072602203 cites W1513618424 @default.
• W2072602203 cites W1548133236 @default.
• W2072602203 cites W1559567442 @default.
• W2072602203 cites W1596024134 @default.
• W2072602203 cites W1964895626 @default.
• W2072602203 cites W1968610711 @default.
• W2072602203 cites W1973838650 @default.
• W2072602203 cites W1973996769 @default.
• W2072602203 cites W1989212333 @default.
• W2072602203 cites W2011071927 @default.
• W2072602203 cites W2011920499 @default.
• W2072602203 cites W2016149358 @default.
• W2072602203 cites W2042314330 @default.
• W2072602203 cites W2052399562 @default.
• W2072602203 cites W2057421063 @default.
• W2072602203 cites W2072249347 @default.
• W2072602203 cites W2078025314 @default.
• W2072602203 cites W2094008335 @default.
• W2072602203 cites W2095000854 @default.
• W2072602203 cites W2103268902 @default.
• W2072602203 cites W2103814083 @default.
• W2072602203 cites W2105776281 @default.
• W2072602203 cites W2106608030 @default.
• W2072602203 cites W2111765237 @default.
• W2072602203 cites W2114104545 @default.
• W2072602203 cites W2114504176 @default.
• W2072602203 cites W2116166762 @default.
• W2072602203 cites W2119149290 @default.
• W2072602203 cites W2120865735 @default.
• W2072602203 cites W2121211805 @default.
• W2072602203 cites W2124985265 @default.
• W2072602203 cites W2141425631 @default.
• W2072602203 cites W2146374277 @default.
• W2072602203 cites W2149541458 @default.
• W2072602203 cites W2153230373 @default.
• W2072602203 cites W2154431984 @default.
• W2072602203 cites W2154537630 @default.
• W2072602203 cites W2155804510 @default.
• W2072602203 cites W2156631105 @default.
• W2072602203 cites W2159485713 @default.
• W2072602203 cites W2160164696 @default.
• W2072602203 cites W2165801297 @default.
• W2072602203 cites W2170074416 @default.
• W2072602203 cites W2170989872 @default.
• W2072602203 cites W2801490189 @default.
• W2072602203 cites W4230096730 @default.
• W2072602203 doi "https://doi.org/10.1371/journal.pone.0017820" @default.
• W2072602203 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3063777" @default.
• W2072602203 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21455293" @default.
• W2072602203 hasPublicationYear "2011" @default.
• W2072602203 type Work @default.
• W2072602203 sameAs 2072602203 @default.
• W2072602203 citedByCount "205" @default.
• W2072602203 countsByYear W20726022032012 @default.
• W2072602203 countsByYear W20726022032013 @default.
• W2072602203 countsByYear W20726022032014 @default.
• W2072602203 countsByYear W20726022032015 @default.
• W2072602203 countsByYear W20726022032016 @default.
• W2072602203 countsByYear W20726022032017 @default.
• W2072602203 countsByYear W20726022032018 @default.
• W2072602203 countsByYear W20726022032019 @default.
• W2072602203 countsByYear W20726022032020 @default.
• W2072602203 countsByYear W20726022032021 @default.
• W2072602203 countsByYear W20726022032022 @default.
• W2072602203 countsByYear W20726022032023 @default.
• W2072602203 crossrefType "journal-article" @default.
• W2072602203 hasAuthorship W2072602203A5000665006 @default.
• W2072602203 hasAuthorship W2072602203A5007067790 @default.
• W2072602203 hasAuthorship W2072602203A5010388465 @default.
• W2072602203 hasAuthorship W2072602203A5010485441 @default.
• W2072602203 hasAuthorship W2072602203A5029270987 @default.
• W2072602203 hasAuthorship W2072602203A5033173474 @default.
• W2072602203 hasAuthorship W2072602203A5061516412 @default.
• W2072602203 hasAuthorship W2072602203A5066373576 @default.
• W2072602203 hasAuthorship W2072602203A5072087302 @default.
• W2072602203 hasAuthorship W2072602203A5074640629 @default.
• W2072602203 hasBestOaLocation W20726022031 @default.
• W2072602203 hasConcept C104317684 @default.
• W2072602203 hasConcept C107397762 @default.
• W2072602203 hasConcept C132917006 @default.
• W2072602203 hasConcept C141231307 @default.
• W2072602203 hasConcept C141674004 @default.
• W2072602203 hasConcept C150194340 @default.

• next