FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072681889> ?p ?o ?g. }

• W2072681889 endingPage "229" @default.
• W2072681889 startingPage "221" @default.
• W2072681889 abstract "The circulatory inflammatory cytokine tumor necrosis factor alpha (TNF-α) is increased in pathologic conditions that initiate or exacerbate vascular endothelial injury, such as diabetes. Protein kinase C (PKC) has been shown to play a critical role in TNF-α-induced human endothelial cell apoptosis. However, the relative roles played by specific isoforms of PKC in TNF-α-induced human endothelial cell apoptosis have not been addressed. We investigated the effects of a selective PKCβ 2 inhibitor (CGP53353) on TNF-α-induced apoptosis in human vascular endothelial cells (cell line ECV304) and on the production of reactive oxygen species and nitric oxide, and compared its effects with rottlerin, a reagent that has been shown to reduce PKCδ protein levels. Cultured human vascular endothelial cells (ECV304) were treated for 24 h with one of 4 regimes: 40 ng/mL TNF-α alone (TNF-α), TNF-α with 10 µmol/L rottlerin (T+rottlerin), TNF-α with 1 µmol/L CGP53353 (T+CGP), or untreated (control). Cell viability was measured by MTT assay, and cell apoptosis was assessed by flow cytometry. TNF-α-induced endothelial cell apoptosis was associated with dramatic increases in production of intracellular hydrogen peroxide (approximately 20 times greater than control) and superoxide (approximately 16 times greater than control), as measured by dichlorofluorescein and dihydroethidium fluorescent staining, respectively. This increase was accompanied by reduced activity of superoxide dismutase and glutathione peroxidase and, subsequently, an increase in the lipid peroxidation product malondialdehyde. CGP53353, but not rottlerin, abolished or attenuated all these changes. We conclude that PKCβ 2 plays a major role in TNF-α-induced human vascular endothelial cell apoptosis." @default.
• W2072681889 created "2016-06-24" @default.
• W2072681889 creator A5003521060 @default.
• W2072681889 creator A5004837837 @default.
• W2072681889 creator A5023090667 @default.
• W2072681889 creator A5027748348 @default.
• W2072681889 creator A5054909887 @default.
• W2072681889 creator A5075622190 @default.
• W2072681889 date "2009-03-01" @default.
• W2072681889 modified "2023-10-14" @default.
• W2072681889 title "Role of protein kinase C β₂activation in TNF-α-induced human vascular endothelial cell apoptosis" @default.
• W2072681889 cites W1591129275 @default.
• W2072681889 cites W1965057305 @default.
• W2072681889 cites W1970391768 @default.
• W2072681889 cites W1979129905 @default.
• W2072681889 cites W1982528727 @default.
• W2072681889 cites W1990792990 @default.
• W2072681889 cites W1998791655 @default.
• W2072681889 cites W2001306570 @default.
• W2072681889 cites W2006683721 @default.
• W2072681889 cites W2007671598 @default.
• W2072681889 cites W2011477764 @default.
• W2072681889 cites W2014616992 @default.
• W2072681889 cites W2030811134 @default.
• W2072681889 cites W2031836453 @default.
• W2072681889 cites W2032259403 @default.
• W2072681889 cites W2038989898 @default.
• W2072681889 cites W2039827702 @default.
• W2072681889 cites W2048186369 @default.
• W2072681889 cites W2055313154 @default.
• W2072681889 cites W2065691148 @default.
• W2072681889 cites W2069018826 @default.
• W2072681889 cites W2075951376 @default.
• W2072681889 cites W2076743846 @default.
• W2072681889 cites W2084863663 @default.
• W2072681889 cites W2090282820 @default.
• W2072681889 cites W2094057901 @default.
• W2072681889 cites W2100864427 @default.
• W2072681889 cites W2104769989 @default.
• W2072681889 cites W2124007377 @default.
• W2072681889 cites W2131090459 @default.
• W2072681889 cites W2151205576 @default.
• W2072681889 cites W2157944478 @default.
• W2072681889 cites W2168948436 @default.
• W2072681889 cites W4237617626 @default.
• W2072681889 doi "https://doi.org/10.1139/y09-004" @default.
• W2072681889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19295663" @default.
• W2072681889 hasPublicationYear "2009" @default.
• W2072681889 type Work @default.
• W2072681889 sameAs 2072681889 @default.
• W2072681889 citedByCount "15" @default.
• W2072681889 countsByYear W20726818892012 @default.
• W2072681889 countsByYear W20726818892013 @default.
• W2072681889 countsByYear W20726818892015 @default.
• W2072681889 countsByYear W20726818892016 @default.
• W2072681889 countsByYear W20726818892018 @default.
• W2072681889 countsByYear W20726818892021 @default.
• W2072681889 countsByYear W20726818892022 @default.
• W2072681889 crossrefType "journal-article" @default.
• W2072681889 hasAuthorship W2072681889A5003521060 @default.
• W2072681889 hasAuthorship W2072681889A5004837837 @default.
• W2072681889 hasAuthorship W2072681889A5023090667 @default.
• W2072681889 hasAuthorship W2072681889A5027748348 @default.
• W2072681889 hasAuthorship W2072681889A5054909887 @default.
• W2072681889 hasAuthorship W2072681889A5075622190 @default.
• W2072681889 hasConcept C123012128 @default.
• W2072681889 hasConcept C134018914 @default.
• W2072681889 hasConcept C153911025 @default.
• W2072681889 hasConcept C17991360 @default.
• W2072681889 hasConcept C184235292 @default.
• W2072681889 hasConcept C185592680 @default.
• W2072681889 hasConcept C190283241 @default.
• W2072681889 hasConcept C195794163 @default.
• W2072681889 hasConcept C202751555 @default.
• W2072681889 hasConcept C2775838275 @default.
• W2072681889 hasConcept C2776151105 @default.
• W2072681889 hasConcept C2776992346 @default.
• W2072681889 hasConcept C2778769476 @default.
• W2072681889 hasConcept C48349386 @default.
• W2072681889 hasConcept C53227056 @default.
• W2072681889 hasConcept C55493867 @default.
• W2072681889 hasConcept C86803240 @default.
• W2072681889 hasConcept C95444343 @default.
• W2072681889 hasConceptScore W2072681889C123012128 @default.
• W2072681889 hasConceptScore W2072681889C134018914 @default.
• W2072681889 hasConceptScore W2072681889C153911025 @default.
• W2072681889 hasConceptScore W2072681889C17991360 @default.
• W2072681889 hasConceptScore W2072681889C184235292 @default.
• W2072681889 hasConceptScore W2072681889C185592680 @default.
• W2072681889 hasConceptScore W2072681889C190283241 @default.
• W2072681889 hasConceptScore W2072681889C195794163 @default.
• W2072681889 hasConceptScore W2072681889C202751555 @default.
• W2072681889 hasConceptScore W2072681889C2775838275 @default.
• W2072681889 hasConceptScore W2072681889C2776151105 @default.
• W2072681889 hasConceptScore W2072681889C2776992346 @default.
• W2072681889 hasConceptScore W2072681889C2778769476 @default.
• W2072681889 hasConceptScore W2072681889C48349386 @default.
• W2072681889 hasConceptScore W2072681889C53227056 @default.

• next