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- W2072719828 abstract "A modified purification is described for an enzyme, from Escherichia coli B, which polymerizes deoxyribonucleoside-5 ′ diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligo-nucleotide primer. At all stages, the enzyme activity copurified with the activity which will polymerize adenosine-5 ′ diphosphate (polynucleotide phosphorylase). Studies of heat stability, the effect of various temperatures of reaction and of disc gel electrophoresis failed to provide evidence that the two activities are separable." @default.
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- W2072719828 date "1974-01-01" @default.
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- W2072719828 title "Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme" @default.
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- W2072719828 doi "https://doi.org/10.1093/nar/1.12.1631" @default.
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