Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072775705> ?p ?o ?g. }
- W2072775705 endingPage "1261" @default.
- W2072775705 startingPage "1253" @default.
- W2072775705 abstract "We have previously reported cyanide at concentrations of up to 150 μM in the sputum of cystic fibrosis patients infected with Pseudomonas aeruginosa and a negative correlation with lung function. Our aim was to investigate possible mechanisms for this association, focusing on the effect of pathophysiologically relevant cyanide levels on human respiratory cell function. Ciliary beat frequency measurements were performed on nasal brushings and nasal air-liquid interface (ALI) cultures obtained from healthy volunteers and cystic fibrosis patients. Potassium cyanide decreased ciliary beat frequency in healthy nasal brushings (n = 6) after 60 min (150 μM: 47% fall, p<0.0012; 75 μM: 32% fall, p<0.0001). Samples from cystic fibrosis patients (n = 3) showed similar results (150 μM: 55% fall, p = 0.001). Ciliary beat frequency inhibition was not due to loss of cell viability and was reversible. The inhibitory mechanism was independent of ATP levels. KCN also significantly inhibited ciliary beat frequency in ALI cultures, albeit to a lesser extent. Ciliary beat frequency measurements on ALI cultures treated with culture supernatants from P. aeruginosa mutants defective in virulence factor production implicated cyanide as a key component inhibiting the ciliary beat frequency. If cyanide production similarly impairs mucocilliary clearance in vivo, it could explain the link with increased disease severity observed in cystic fibrosis patients with detectable cyanide in their airway." @default.
- W2072775705 created "2016-06-24" @default.
- W2072775705 creator A5011439043 @default.
- W2072775705 creator A5012579486 @default.
- W2072775705 creator A5024588878 @default.
- W2072775705 creator A5032064034 @default.
- W2072775705 creator A5042275688 @default.
- W2072775705 creator A5062776492 @default.
- W2072775705 creator A5063513802 @default.
- W2072775705 creator A5067795136 @default.
- W2072775705 creator A5082797668 @default.
- W2072775705 date "2014-09-03" @default.
- W2072775705 modified "2023-10-02" @default.
- W2072775705 title "Cyanide levels found in infected cystic fibrosis sputum inhibit airway ciliary function" @default.
- W2072775705 cites W1567281461 @default.
- W2072775705 cites W1786021994 @default.
- W2072775705 cites W1941590970 @default.
- W2072775705 cites W1943184335 @default.
- W2072775705 cites W1966979140 @default.
- W2072775705 cites W1978050919 @default.
- W2072775705 cites W1998555308 @default.
- W2072775705 cites W2000051984 @default.
- W2072775705 cites W2001444438 @default.
- W2072775705 cites W2001735366 @default.
- W2072775705 cites W2019484771 @default.
- W2072775705 cites W2023456873 @default.
- W2072775705 cites W2026100561 @default.
- W2072775705 cites W2031025878 @default.
- W2072775705 cites W2049293484 @default.
- W2072775705 cites W2070383383 @default.
- W2072775705 cites W2070432566 @default.
- W2072775705 cites W2078133892 @default.
- W2072775705 cites W2100669918 @default.
- W2072775705 cites W2101735704 @default.
- W2072775705 cites W2103367484 @default.
- W2072775705 cites W2106509824 @default.
- W2072775705 cites W2108149817 @default.
- W2072775705 cites W2118929607 @default.
- W2072775705 cites W2125164964 @default.
- W2072775705 cites W2125486571 @default.
- W2072775705 cites W2130951311 @default.
- W2072775705 cites W2132354417 @default.
- W2072775705 cites W2133653432 @default.
- W2072775705 cites W2144026573 @default.
- W2072775705 cites W2145163797 @default.
- W2072775705 cites W2146331405 @default.
- W2072775705 cites W2149658463 @default.
- W2072775705 cites W2155411515 @default.
- W2072775705 cites W2155932530 @default.
- W2072775705 cites W2336150417 @default.
- W2072775705 cites W2475269388 @default.
- W2072775705 cites W4230982340 @default.
- W2072775705 doi "https://doi.org/10.1183/09031936.00097014" @default.
- W2072775705 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25186256" @default.
- W2072775705 hasPublicationYear "2014" @default.
- W2072775705 type Work @default.
- W2072775705 sameAs 2072775705 @default.
- W2072775705 citedByCount "26" @default.
- W2072775705 countsByYear W20727757052015 @default.
- W2072775705 countsByYear W20727757052016 @default.
- W2072775705 countsByYear W20727757052017 @default.
- W2072775705 countsByYear W20727757052018 @default.
- W2072775705 countsByYear W20727757052019 @default.
- W2072775705 countsByYear W20727757052020 @default.
- W2072775705 countsByYear W20727757052021 @default.
- W2072775705 countsByYear W20727757052022 @default.
- W2072775705 countsByYear W20727757052023 @default.
- W2072775705 crossrefType "journal-article" @default.
- W2072775705 hasAuthorship W2072775705A5011439043 @default.
- W2072775705 hasAuthorship W2072775705A5012579486 @default.
- W2072775705 hasAuthorship W2072775705A5024588878 @default.
- W2072775705 hasAuthorship W2072775705A5032064034 @default.
- W2072775705 hasAuthorship W2072775705A5042275688 @default.
- W2072775705 hasAuthorship W2072775705A5062776492 @default.
- W2072775705 hasAuthorship W2072775705A5063513802 @default.
- W2072775705 hasAuthorship W2072775705A5067795136 @default.
- W2072775705 hasAuthorship W2072775705A5082797668 @default.
- W2072775705 hasBestOaLocation W20727757051 @default.
- W2072775705 hasConcept C100094513 @default.
- W2072775705 hasConcept C126322002 @default.
- W2072775705 hasConcept C134018914 @default.
- W2072775705 hasConcept C142724271 @default.
- W2072775705 hasConcept C179104552 @default.
- W2072775705 hasConcept C185592680 @default.
- W2072775705 hasConcept C2776301714 @default.
- W2072775705 hasConcept C2776424019 @default.
- W2072775705 hasConcept C2776938444 @default.
- W2072775705 hasConcept C2777637488 @default.
- W2072775705 hasConcept C2777714996 @default.
- W2072775705 hasConcept C2778206077 @default.
- W2072775705 hasConcept C2778455166 @default.
- W2072775705 hasConcept C2779379686 @default.
- W2072775705 hasConcept C2781069245 @default.
- W2072775705 hasConcept C523546767 @default.
- W2072775705 hasConcept C54355233 @default.
- W2072775705 hasConcept C71924100 @default.
- W2072775705 hasConcept C86803240 @default.
- W2072775705 hasConcept C95444343 @default.