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- W2072779644 abstract "Methodology is described for the synthesis of DNA oligomers containing a free 3′-thiol group which can be selectively crosslinked with a wide variety of probes. This chemistry is compatible with both phosphotriester and phosphoramidite solid phase chemistry. Moreover, the sulphydryl group is introduced into the 3′-nucleoside solid support linkage prior to oligonucleotide synthesis. Consequently, no additional coupling steps are required after oligonucleotide synthesis, and isolation of the 3′-thiol oligonucleotide requires only one additional deprotection step. Cross-linking of the thiol-containing oligonucleotide to a fluorescent probe was carried out with high selectivity, in high yield, and under mild conditions." @default.
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- W2072779644 date "1987-01-01" @default.
- W2072779644 modified "2023-10-18" @default.
- W2072779644 title "Efficient methods for attachment of thiol specific probes to the 3′-ends of synthetic oligodeoxyribonucleotides" @default.
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- W2072779644 doi "https://doi.org/10.1093/nar/15.13.5305" @default.
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