FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072812437> ?p ?o ?g. }

• W2072812437 endingPage "4372" @default.
• W2072812437 startingPage "4361" @default.
• W2072812437 abstract "The chloroplast-encoded psbA gene encodes the D1 polypeptide of the photosystem II reaction center, which is synthesized at high rates in the light. In Chlamydomonas reinhardtii, the psbA gene contains four self-splicing group I introns whose rates of splicing in vivo are increased at least 6-10-fold by light. However, because psbA is an abundant mRNA, and some chloroplast mRNAs appear to be in great excess of what is needed to sustain translation rates, the developmental significance of light-promoted splicing has not been clear. To address this and other questions, potentially destabilizing substitutions were made in several predicted helices of the fourth psbA intron, Cr.psbA4, and their effects on in vitro and in vivo splicing assessed. Two-nucleotide substitutions in P4 and P7 were necessary to substantially reduce splicing of this intron in vivo, although most mutations reduced self-splicing in vitro. The P7-4,5 mutant, whose splicing was completely blocked, showed no photoautotrophic growth and synthesis of a truncated D1 (exons 1-4) polypeptide from the unspliced mRNA. Most informative was the P4'-3,4 mutant, which exhibited a 45% reduction in spliced psbA mRNA, a 28% reduction in synthesis of full-length D1, and an 18% reduction in photoautotrophic growth. These results indicate that psbA mRNA is not in great excess, and that highly efficient splicing of psbA introns, which is afforded by light conditions, is necessary for optimal photosynthetic growth." @default.
• W2072812437 created "2016-06-24" @default.
• W2072812437 creator A5067841853 @default.
• W2072812437 creator A5070821846 @default.
• W2072812437 date "2003-08-01" @default.
• W2072812437 modified "2023-09-25" @default.
• W2072812437 title "Mutagenesis of a light-regulated psbA intron reveals the importance of efficient splicing for photosynthetic growth" @default.
• W2072812437 cites W1485772597 @default.
• W2072812437 cites W1531324966 @default.
• W2072812437 cites W1565090424 @default.
• W2072812437 cites W1584262500 @default.
• W2072812437 cites W1589115497 @default.
• W2072812437 cites W1618072718 @default.
• W2072812437 cites W1853431358 @default.
• W2072812437 cites W1967908729 @default.
• W2072812437 cites W1974126908 @default.
• W2072812437 cites W1987470529 @default.
• W2072812437 cites W1988908524 @default.
• W2072812437 cites W1994223718 @default.
• W2072812437 cites W2000738377 @default.
• W2072812437 cites W2002688220 @default.
• W2072812437 cites W2005603560 @default.
• W2072812437 cites W2014406371 @default.
• W2072812437 cites W2014431045 @default.
• W2072812437 cites W2014756276 @default.
• W2072812437 cites W2018289835 @default.
• W2072812437 cites W2033934723 @default.
• W2072812437 cites W2035783320 @default.
• W2072812437 cites W2039263723 @default.
• W2072812437 cites W2043010766 @default.
• W2072812437 cites W2046942074 @default.
• W2072812437 cites W2057341432 @default.
• W2072812437 cites W2064494727 @default.
• W2072812437 cites W2066948049 @default.
• W2072812437 cites W2070619414 @default.
• W2072812437 cites W2076178582 @default.
• W2072812437 cites W2087288233 @default.
• W2072812437 cites W2089496339 @default.
• W2072812437 cites W2096810136 @default.
• W2072812437 cites W2098844574 @default.
• W2072812437 cites W2100837269 @default.
• W2072812437 cites W2104156460 @default.
• W2072812437 cites W2105585246 @default.
• W2072812437 cites W2119917563 @default.
• W2072812437 cites W2122503501 @default.
• W2072812437 cites W2124529933 @default.
• W2072812437 cites W2128085055 @default.
• W2072812437 cites W2131048161 @default.
• W2072812437 cites W2141228121 @default.
• W2072812437 cites W2164423690 @default.
• W2072812437 cites W2167421006 @default.
• W2072812437 cites W2576734941 @default.
• W2072812437 cites W332962347 @default.
• W2072812437 cites W90833451 @default.
• W2072812437 doi "https://doi.org/10.1093/nar/gkg643" @default.
• W2072812437 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/169925" @default.
• W2072812437 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12888495" @default.
• W2072812437 hasPublicationYear "2003" @default.
• W2072812437 type Work @default.
• W2072812437 sameAs 2072812437 @default.
• W2072812437 citedByCount "17" @default.
• W2072812437 countsByYear W20728124372012 @default.
• W2072812437 countsByYear W20728124372014 @default.
• W2072812437 countsByYear W20728124372019 @default.
• W2072812437 countsByYear W20728124372022 @default.
• W2072812437 crossrefType "journal-article" @default.
• W2072812437 hasAuthorship W2072812437A5067841853 @default.
• W2072812437 hasAuthorship W2072812437A5070821846 @default.
• W2072812437 hasBestOaLocation W20728124371 @default.
• W2072812437 hasConcept C104317684 @default.
• W2072812437 hasConcept C143065580 @default.
• W2072812437 hasConcept C153911025 @default.
• W2072812437 hasConcept C2776580309 @default.
• W2072812437 hasConcept C2777727519 @default.
• W2072812437 hasConcept C36823959 @default.
• W2072812437 hasConcept C42576000 @default.
• W2072812437 hasConcept C54355233 @default.
• W2072812437 hasConcept C54458228 @default.
• W2072812437 hasConcept C67705224 @default.
• W2072812437 hasConcept C86803240 @default.
• W2072812437 hasConcept C94671646 @default.
• W2072812437 hasConceptScore W2072812437C104317684 @default.
• W2072812437 hasConceptScore W2072812437C143065580 @default.
• W2072812437 hasConceptScore W2072812437C153911025 @default.
• W2072812437 hasConceptScore W2072812437C2776580309 @default.
• W2072812437 hasConceptScore W2072812437C2777727519 @default.
• W2072812437 hasConceptScore W2072812437C36823959 @default.
• W2072812437 hasConceptScore W2072812437C42576000 @default.
• W2072812437 hasConceptScore W2072812437C54355233 @default.
• W2072812437 hasConceptScore W2072812437C54458228 @default.
• W2072812437 hasConceptScore W2072812437C67705224 @default.
• W2072812437 hasConceptScore W2072812437C86803240 @default.
• W2072812437 hasConceptScore W2072812437C94671646 @default.
• W2072812437 hasIssue "15" @default.
• W2072812437 hasLocation W20728124371 @default.
• W2072812437 hasLocation W20728124372 @default.
• W2072812437 hasLocation W20728124373 @default.
• W2072812437 hasOpenAccess W2072812437 @default.

• next