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- W2072826763 abstract "We report a new approach for the determination of influence of temperature on enzymatic activity within a single fixed cell. We investigate enzyme activity at different temperature (22, 27, 32,37 and 42°C) by means of directed infrared laser heating. Our method generates individual cells at selected location, through the use of a flexible microfluidic delivery device and IR fiber heating system. Using timed exposure to the pore-forming agent digitonin, we successfully controlled the extent of poration within the plasma membrane of individual NG-108 cells. Mildly permeabilized cells (∼100 pores) were exposed to substrate concentrations for alkaline phosphotase and exposure to a series of temperature. We generated quantitative estimates for intracellular enzyme activity at different temperature at the single-cell level. This rapid approach offers a new methodology for characterizing influence of temperature on enzyme activity within single cells." @default.
- W2072826763 created "2016-06-24" @default.
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- W2072826763 date "2013-01-01" @default.
- W2072826763 modified "2023-09-30" @default.
- W2072826763 title "Influence of Temperature on Alkaline Phosphatase Activity in Single Cells" @default.
- W2072826763 doi "https://doi.org/10.1016/j.bpj.2012.11.3731" @default.
- W2072826763 hasPublicationYear "2013" @default.
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