FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072907135> ?p ?o ?g. }

• W2072907135 endingPage "594" @default.
• W2072907135 startingPage "587" @default.
• W2072907135 abstract "Transgenic mice are increasingly used as animal models for studies of gene function and regulation of mammalian genes. Although there has been continuous and remarkable progress in the development of transgenic technology over several decades, many aspects of the resulting transgenic model's phenotype cannot be completely predicted. For example, it is well known that as a consequence of the random insertion of the injected DNA construct, several founder mice of the new line need to be analyzed for possible differences in phenotype secondary to different insertion sites. The Knock out technique for transgenic production disrupts a specific gene by insertion or homologous recombination creating a null expression or replacement of the gene with a marker to localize it expression. This modification could result in pleiotropic phenotype if the gene is also expressed in tissues other than the target organs. Although the future breeding performance of the newly created model is critical to many studies, it is rarely anticipated that the new integrations could modify the reproductive profile of the new transgenic line. To date, few studies have demonstrated the difference between the parent strain's reproductive performance and the newly developed transgenic model. This study was designed to determine whether a genetic modification, knock out (KO) or transgenics, not anticipated to affect reproductive performance could affect the resulting reproductive profile of the newly developed transgenic mouse. More specifically, this study is designed to study the impact of the genetic modification on the ability of gametes to be fertilized in vitro. We analyzed the reproductive performance of mice with different background strains: FVB/N, C57BL/6 (129Sv/J x C57Bl/6)F1 and outbred CD1((R)) and compared them to mice of the same strain carrying a transgene or KO which was not anticipated to affect fertility. In vitro Fertilization was used to analyze the fertility of the mice. Oocytes from superovulated females were inseminated with sperm of same background. Fertility rate was considered as the percentage of two cell embryos scored 24 h after insemination. The data collected from this study shows that the fertilization rate is affected (reduced to half fold) in some of the transgenic mice compared to the respective Wild Type (WT) mice. For the WT the average fertility rate ranged from 80% (C57BL/6), 90% (FVB/N), 45% (129Sv/J x C57Bl/6)F1 and 43% (CD1). For transgenic mice it was 52% (C57BL/6), 65% (FVB/N), 22% (129Sv/J x C57Bl/6)F1 and 25% (CD1)." @default.
• W2072907135 created "2016-06-24" @default.
• W2072907135 creator A5021225131 @default.
• W2072907135 creator A5022149506 @default.
• W2072907135 creator A5071696325 @default.
• W2072907135 date "2009-10-21" @default.
• W2072907135 modified "2023-09-23" @default.
• W2072907135 title "Fertility comparison between wild type and transgenic mice by in vitro fertilization" @default.
• W2072907135 cites W1587435244 @default.
• W2072907135 cites W1853421264 @default.
• W2072907135 cites W1968283357 @default.
• W2072907135 cites W1968458490 @default.
• W2072907135 cites W1973862215 @default.
• W2072907135 cites W1974465181 @default.
• W2072907135 cites W1982511069 @default.
• W2072907135 cites W1998527032 @default.
• W2072907135 cites W2014002841 @default.
• W2072907135 cites W2016697658 @default.
• W2072907135 cites W2024462833 @default.
• W2072907135 cites W2031781918 @default.
• W2072907135 cites W2033688342 @default.
• W2072907135 cites W2034887388 @default.
• W2072907135 cites W2035337493 @default.
• W2072907135 cites W2038725731 @default.
• W2072907135 cites W2057641424 @default.
• W2072907135 cites W2057698095 @default.
• W2072907135 cites W2058319711 @default.
• W2072907135 cites W2058469411 @default.
• W2072907135 cites W2064848005 @default.
• W2072907135 cites W2065749107 @default.
• W2072907135 cites W2066194839 @default.
• W2072907135 cites W2069433703 @default.
• W2072907135 cites W2075240053 @default.
• W2072907135 cites W2075797310 @default.
• W2072907135 cites W2077364768 @default.
• W2072907135 cites W2079447068 @default.
• W2072907135 cites W2084394846 @default.
• W2072907135 cites W2084632723 @default.
• W2072907135 cites W2093433084 @default.
• W2072907135 cites W2095347611 @default.
• W2072907135 cites W2095887553 @default.
• W2072907135 cites W2107670036 @default.
• W2072907135 cites W2120473761 @default.
• W2072907135 cites W2126616586 @default.
• W2072907135 cites W2130688888 @default.
• W2072907135 cites W2145823842 @default.
• W2072907135 cites W2161683708 @default.
• W2072907135 cites W2167473320 @default.
• W2072907135 cites W4234724519 @default.
• W2072907135 doi "https://doi.org/10.1007/s11248-009-9336-2" @default.
• W2072907135 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3110660" @default.
• W2072907135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19844803" @default.
• W2072907135 hasPublicationYear "2009" @default.
• W2072907135 type Work @default.
• W2072907135 sameAs 2072907135 @default.
• W2072907135 citedByCount "26" @default.
• W2072907135 countsByYear W20729071352012 @default.
• W2072907135 countsByYear W20729071352013 @default.
• W2072907135 countsByYear W20729071352014 @default.
• W2072907135 countsByYear W20729071352015 @default.
• W2072907135 countsByYear W20729071352016 @default.
• W2072907135 countsByYear W20729071352017 @default.
• W2072907135 countsByYear W20729071352018 @default.
• W2072907135 countsByYear W20729071352021 @default.
• W2072907135 crossrefType "journal-article" @default.
• W2072907135 hasAuthorship W2072907135A5021225131 @default.
• W2072907135 hasAuthorship W2072907135A5022149506 @default.
• W2072907135 hasAuthorship W2072907135A5071696325 @default.
• W2072907135 hasBestOaLocation W20729071352 @default.
• W2072907135 hasConcept C102230213 @default.
• W2072907135 hasConcept C102744134 @default.
• W2072907135 hasConcept C104317684 @default.
• W2072907135 hasConcept C111829913 @default.
• W2072907135 hasConcept C127716648 @default.
• W2072907135 hasConcept C141035611 @default.
• W2072907135 hasConcept C54355233 @default.
• W2072907135 hasConcept C86803240 @default.
• W2072907135 hasConcept C95444343 @default.
• W2072907135 hasConceptScore W2072907135C102230213 @default.
• W2072907135 hasConceptScore W2072907135C102744134 @default.
• W2072907135 hasConceptScore W2072907135C104317684 @default.
• W2072907135 hasConceptScore W2072907135C111829913 @default.
• W2072907135 hasConceptScore W2072907135C127716648 @default.
• W2072907135 hasConceptScore W2072907135C141035611 @default.
• W2072907135 hasConceptScore W2072907135C54355233 @default.
• W2072907135 hasConceptScore W2072907135C86803240 @default.
• W2072907135 hasConceptScore W2072907135C95444343 @default.
• W2072907135 hasIssue "4" @default.
• W2072907135 hasLocation W20729071351 @default.
• W2072907135 hasLocation W20729071352 @default.
• W2072907135 hasLocation W20729071353 @default.
• W2072907135 hasLocation W20729071354 @default.
• W2072907135 hasOpenAccess W2072907135 @default.
• W2072907135 hasPrimaryLocation W20729071351 @default.
• W2072907135 hasRelatedWork W1965447352 @default.
• W2072907135 hasRelatedWork W1983634938 @default.
• W2072907135 hasRelatedWork W2064812551 @default.
• W2072907135 hasRelatedWork W2072907135 @default.

• next