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• W2072933469 abstract "On incubation of HeLa cells in chilled isotonic medium, intracellular Na+ (Nac+) increased and K+ (Kc+) decreased with time, reaching steady levels after 3 h. The steady levels varied in parallel with the extracellular cation concentrations ([Na+]e, [K+]e). The cell volumes and the protein and water contents, respectively, of cells kept for 3 h in chilled media of various [Na+]e and [K+]e were not significantly different. Ouabain-sensitive Rb+ influx took place at the initial rate for a certain period which depended on [Na+]c at the beginning of the assays. The existence of two external K+ loading sites per Na+/K+-pump was demonstrated. The affinities of the sites for Rb+ as a congener of K+ were almost the same. Na+e inhibited ouabain-sensitive Rb+ influx competitively, whereas K+ was not inhibitory. Kinetic parameters were determined: the K 1/2 for Rbe+ in the absence of Na+e was 0.16 mM and th Ki for Na+e was 36.8 mM; the K 1/2 for Na+c was 19.5 mM and the Ki for K+c seemed to be extremely large. The rate equation of the ouabain-sensitive Rb+ influx suggests that Na+ and K+ are exchanged alternately through the pump by a binary mechanism." @default.
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• W2072933469 date "1984-09-01" @default.
• W2072933469 modified "2023-09-27" @default.
• W2072933469 title "Kinetic parameters and mechanism of active cation transport in HeLa cells as studied by Rb+ influx" @default.
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• W2072933469 doi "https://doi.org/10.1016/0005-2736(84)90184-6" @default.
• W2072933469 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6466673" @default.
• W2072933469 hasPublicationYear "1984" @default.
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