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- W2072947140 abstract "Abstract Saccharomyces cerevisiae invertase, chemically modified with chitosan, was immobilized on a carboxymethylcellulose‐coated chitin support via polyelectrolyte complex formation. The yield of immobilized protein was determined to be 72% and the enzyme retained 68% of the initial invertase activity. The optimum temperature for invertase was increased by 5°C and its thermostability was enhanced by about 9°C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength solutions and was 12.6‐fold more resistant to thermal treatment at 65°C than the native counterpart. The prepared biocatalyst retained 98% and 100% of the original catalytic activity after 10 cycles of reuse and 70 h of continuous operational regime in a packed bed reactor, respectively. The immobilized enzyme retained 95% of its activity after 50 days of storage at 37°C." @default.
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- W2072947140 date "2006-09-01" @default.
- W2072947140 modified "2023-09-23" @default.
- W2072947140 title "Immobilization of Chitosan‐Invertase Neoglycoconjugate on Carboxymethylcellulose‐Modified Chitin" @default.
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- W2072947140 doi "https://doi.org/10.1080/10826060600716695" @default.
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