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- W2073022365 endingPage "1636" @default.
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- W2073022365 abstract "Interleukin 17 (IL17) is now known to be involved in a number of chronic inflammatory disorders. However, the mechanisms regulating its production in inflammatory bowel disease (IBD) are still unclear.Endoscopic biopsies or surgical specimens were taken from inflamed and uninflamed colonic mucosa of 72 patients with IBD (38 with Crohn's disease and 34 with ulcerative colitis), and normal colon of 38 control subjects. IL17 and interferon gamma (IFNgamma) were detected by ELISA in the supernatants of biopsies cultured ex vivo, and anti-CD3/CD28-stimulated lamina propria mononuclear cells (LPMCs) incubated with IL12, IL23, IL1beta plus IL6, transforming growth factor beta1 (TGFbeta1), or anti-IL21 neutralising antibody. Intracellular flow cytometry was performed to analyse mucosal Th17 and Th1/Th17 cells.IL17 production by organ culture biopsies was higher in IBD inflamed mucosa than IBD uninflamed mucosa and controls, and was equivalent in amount to IFNgamma. Anti-CD3/CD28-stimulated IBD LPMCs produced higher IL17 amounts compared to controls. The percentages of Th17 and Th1/Th17 cells were increased in patients with IBD. IL23 and IL1beta plus IL6 had no effect on IBD LPMC production of IL17; however, IL12 markedly increased IFNgamma production and decreased IL17 production. TGFbeta1 dose-dependently decreased IFNgamma, but had no significant inhibitory effect on IL17 production. Blocking IL21 significantly downregulated IL17 production.Our findings support a role for IL12, TGFbeta and IL21 in modulating IL17/IFNgamma production in IBD. The abundant IL17 in inflamed IBD mucosa may help explain the relative lack of efficacy of anti-IFNgamma antibodies in clinical trials of Crohn's disease." @default.
- W2073022365 created "2016-06-24" @default.
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- W2073022365 date "2009-09-08" @default.
- W2073022365 modified "2023-10-16" @default.
- W2073022365 title "Differential regulation of interleukin 17 and interferon production in inflammatory bowel disease" @default.
- W2073022365 cites W1500371449 @default.
- W2073022365 cites W1501206772 @default.
- W2073022365 cites W1505685427 @default.
- W2073022365 cites W1523941392 @default.
- W2073022365 cites W1537063289 @default.
- W2073022365 cites W1548249686 @default.
- W2073022365 cites W1565278837 @default.
- W2073022365 cites W1571166414 @default.
- W2073022365 cites W1598899303 @default.
- W2073022365 cites W1637703629 @default.
- W2073022365 cites W1642457845 @default.
- W2073022365 cites W1670573097 @default.
- W2073022365 cites W1744501211 @default.
- W2073022365 cites W1855226200 @default.
- W2073022365 cites W1856119395 @default.
- W2073022365 cites W1895076577 @default.
- W2073022365 cites W1899583926 @default.
- W2073022365 cites W1946093640 @default.
- W2073022365 cites W1963639770 @default.
- W2073022365 cites W1964300433 @default.
- W2073022365 cites W1964345309 @default.
- W2073022365 cites W1967165769 @default.
- W2073022365 cites W1969971297 @default.
- W2073022365 cites W1971708477 @default.
- W2073022365 cites W1971834098 @default.
- W2073022365 cites W1972215874 @default.
- W2073022365 cites W1972386568 @default.
- W2073022365 cites W1972926790 @default.
- W2073022365 cites W1974259787 @default.
- W2073022365 cites W1981006441 @default.
- W2073022365 cites W1981714996 @default.
- W2073022365 cites W1982243301 @default.
- W2073022365 cites W1982926364 @default.
- W2073022365 cites W1983233325 @default.
- W2073022365 cites W1984739754 @default.
- W2073022365 cites W1985669208 @default.
- W2073022365 cites W1987628377 @default.
- W2073022365 cites W1990666294 @default.
- W2073022365 cites W1991124488 @default.
- W2073022365 cites W1992465269 @default.
- W2073022365 cites W1996139830 @default.
- W2073022365 cites W1997638368 @default.
- W2073022365 cites W1997999969 @default.
- W2073022365 cites W1998599033 @default.
- W2073022365 cites W1998774782 @default.
- W2073022365 cites W1999195236 @default.
- W2073022365 cites W1999561404 @default.
- W2073022365 cites W2000024124 @default.
- W2073022365 cites W2000208875 @default.
- W2073022365 cites W2000379621 @default.
- W2073022365 cites W2001645752 @default.
- W2073022365 cites W2004582112 @default.
- W2073022365 cites W2005552681 @default.
- W2073022365 cites W2005553679 @default.
- W2073022365 cites W2006249161 @default.
- W2073022365 cites W2008713912 @default.
- W2073022365 cites W2012680181 @default.
- W2073022365 cites W2014750930 @default.
- W2073022365 cites W2015597393 @default.
- W2073022365 cites W2015788300 @default.
- W2073022365 cites W2017046428 @default.
- W2073022365 cites W2017356934 @default.
- W2073022365 cites W2017443683 @default.
- W2073022365 cites W2018166175 @default.
- W2073022365 cites W2018542503 @default.
- W2073022365 cites W2019931906 @default.
- W2073022365 cites W2020873782 @default.
- W2073022365 cites W2024701119 @default.
- W2073022365 cites W2026058763 @default.
- W2073022365 cites W2027949856 @default.
- W2073022365 cites W2028547258 @default.
- W2073022365 cites W2028935955 @default.
- W2073022365 cites W2030173580 @default.
- W2073022365 cites W2031035500 @default.
- W2073022365 cites W2032218562 @default.
- W2073022365 cites W2033164113 @default.
- W2073022365 cites W2034016444 @default.
- W2073022365 cites W2035551117 @default.
- W2073022365 cites W2036500213 @default.
- W2073022365 cites W2038131331 @default.
- W2073022365 cites W2040113209 @default.
- W2073022365 cites W2040512118 @default.
- W2073022365 cites W2043667001 @default.