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- W2073028175 abstract "Whitmania pigra is common in China and has been used as a traditional Chinese anticoagulant medicine for years, but its effective components are unknown to scientists. In this article we report a rapid method for isolation and purification of an anticoagulant from W. pigra for the first time. An acetone–water extract of W. pigra was subjected to anion-exchange chromatography on a Sephadex DEAE A-50 column, and gel permeation chromatography on Sephadex G-25 and Sephadex LH-20 columns successively, which afforded a fraction with potent anticoagulant activity. An anticoagulant was isolated and purified from this fraction by reversed-phase high-performance liquid chromatography (RP-HPLC). It was identified as a single pure substance by RP-HPLC and sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). This component was named whitmanin and its molecular weight was determined as 8608 Da by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). Copyright © 2007 John Wiley & Sons, Ltd." @default.
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- W2073028175 date "2007-01-01" @default.
- W2073028175 modified "2023-10-18" @default.
- W2073028175 title "A rapid method for isolation and purification of an anticoagulant fromWhitmania pigra" @default.
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- W2073028175 doi "https://doi.org/10.1002/bmc.739" @default.
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