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• W2073033127 abstract "A method for labeling cells with technetium-99m via hydrophilic, polycationic poly d-lysine modified by N-acetyl homocysteine has been developed. The modified polylysine (MPL) is labeled with 99mTc in >95% yield and is stable for >12 h. Maximum cell labeling is achieved by a 1-h incubation at room temperature with isolated leukocytes, granulocytes and peripheral blood mononuclear cells attaining 60–75% 99mTc incorporation, and red blood cells 35%. Ninety-two percent of the label is retained by leukocytes after a 1-h incubation at room temperature in 50% serum. The cell uptake of 99mTc-MPL is affected by the presence of negatively charged species in the medium; the inhibitory effects of 5% serum or serum albumin can be reversed by increasing the concentration of 99mTc-MPL, while those of heparin are not." @default.
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• W2073033127 date "1996-01-01" @default.
• W2073033127 modified "2023-09-25" @default.
• W2073033127 title "A new approach to labeling cells with technetium-99m, part I. Preparation of modified polylysine and in vitro cell labeling" @default.
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• W2073033127 doi "https://doi.org/10.1016/0969-8051(95)02071-3" @default.
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