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- W2073033734 abstract "Abstract It has been suggested by Panet et al. (Virology 114 , 567–572, 1981) that NIH 3T3 cells chronically infected with Moloney murine leukemia virus are deficient in 2',5'-oligo(A dependent endoribonuclease (RNase L). However, we detected RNase L activity in extracts of these NIH 3T3 cells prepared in the presence of the protease inhibitor phenylmethylsulfonyl fluoride. These extracts could bind labeled 2',5'-oligo(A) in a binding assay and specific polypeptides in these extracts could be crosslinked to a periodate-oxidized derivative of 2′,5′-oligo(A). Similar polypeptides were detected in cytoplasmic and nuclear extracts of HeLa cells, which showed RNase L activity even in the absence of added phenylmethylsulfonyl fluoride." @default.
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- W2073033734 date "1982-10-01" @default.
- W2073033734 modified "2023-09-27" @default.
- W2073033734 title "2′,5′-oligo(A)-activated endonuclease in NIH 3T3 mouse cells chronically infected with moloney murine leukemia virus" @default.
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- W2073033734 doi "https://doi.org/10.1016/0042-6822(82)90252-5" @default.
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