Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073049420> ?p ?o ?g. }
- W2073049420 endingPage "312" @default.
- W2073049420 startingPage "303" @default.
- W2073049420 abstract "Background Volatile anesthetics decrease ischemic brain injury. Mechanisms for this protection remain under investigation. The authors hypothesized that volatile anesthetics serve as antioxidants in a neuronal-glial cell culture system. Methods Primary cortical neuronal-glial cultures were prepared from fetal rat brain. Cultures were exposed to iron, H2O2, or xanthine-xanthine oxidase for 30 min in serum-free media containing dissolved isoflurane (0-3.2 mm), sevoflurane (0-3.6 mm), halothane (0-4.1 mm), n-hexanol, or known antioxidants. Cell damage was assessed by release of lactate dehydrogenase (LDH) and trypan blue exclusion 24 h later. Lipid peroxidation was measured by the production of thiobarbituric acid-reactive substances in a cell-free lipid system. Iron and calcium uptake and mitochondrial depolarization were measured after exposure to iron in the presence or absence of isoflurane. Results Deferoxamine reduced LDH release caused by H2O2 or xanthine-xanthine oxidase, but the volatile anesthetics had no effect. Iron-induced LDH release was prevented by the volatile anesthetics (maximum effect for halothane = 1.2 mm, isoflurane = 1.2 mm, and sevoflurane = 2.1 mm aqueous phase). When corrected for lipid solubility, the three volatile anesthetics were equipotent against iron-induced LDH release. In the cell-free system, there was no effect of the anesthetics on thiobarbituric acid-reactive substance formation in contrast to Trolox, which provided complete inhibition. Isoflurane (1.2 mm) reduced mean iron uptake by 46% and inhibited mitochondrial depolarization but had no effect on calcium uptake. Conclusions Volatile anesthetics reduced cell death induced by oxidative stress only in the context of iron challenge. The likely reason for protection against iron toxicity is inhibition of iron uptake and therefore indirect reduction of subsequent intracellular oxidative stress caused by this challenge. These data argue against a primary antioxidant effect of volatile anesthetics." @default.
- W2073049420 created "2016-06-24" @default.
- W2073049420 creator A5031840999 @default.
- W2073049420 creator A5043950964 @default.
- W2073049420 creator A5050506489 @default.
- W2073049420 creator A5056318077 @default.
- W2073049420 creator A5070593854 @default.
- W2073049420 creator A5079187727 @default.
- W2073049420 date "2001-02-01" @default.
- W2073049420 modified "2023-10-16" @default.
- W2073049420 title "Absence of Direct Antioxidant Effects from Volatile Anesthetics in Primary Mixed Neuronal–Glial Cultures" @default.
- W2073049420 cites W103965868 @default.
- W2073049420 cites W1953642784 @default.
- W2073049420 cites W1965427209 @default.
- W2073049420 cites W1977015296 @default.
- W2073049420 cites W1983858227 @default.
- W2073049420 cites W1985859760 @default.
- W2073049420 cites W1990628495 @default.
- W2073049420 cites W1991381764 @default.
- W2073049420 cites W2013385653 @default.
- W2073049420 cites W2022995782 @default.
- W2073049420 cites W2047076446 @default.
- W2073049420 cites W2060791535 @default.
- W2073049420 cites W2065523036 @default.
- W2073049420 cites W2071256185 @default.
- W2073049420 cites W2071397500 @default.
- W2073049420 cites W2077499781 @default.
- W2073049420 cites W2078260486 @default.
- W2073049420 cites W2078488942 @default.
- W2073049420 cites W2080442688 @default.
- W2073049420 cites W2080771497 @default.
- W2073049420 cites W2085273994 @default.
- W2073049420 cites W2086159970 @default.
- W2073049420 cites W2093382900 @default.
- W2073049420 cites W21389353 @default.
- W2073049420 cites W2164164873 @default.
- W2073049420 cites W2314479559 @default.
- W2073049420 doi "https://doi.org/10.1097/00000542-200102000-00021" @default.
- W2073049420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11176096" @default.
- W2073049420 hasPublicationYear "2001" @default.
- W2073049420 type Work @default.
- W2073049420 sameAs 2073049420 @default.
- W2073049420 citedByCount "24" @default.
- W2073049420 countsByYear W20730494202012 @default.
- W2073049420 countsByYear W20730494202014 @default.
- W2073049420 countsByYear W20730494202015 @default.
- W2073049420 countsByYear W20730494202017 @default.
- W2073049420 crossrefType "journal-article" @default.
- W2073049420 hasAuthorship W2073049420A5031840999 @default.
- W2073049420 hasAuthorship W2073049420A5043950964 @default.
- W2073049420 hasAuthorship W2073049420A5050506489 @default.
- W2073049420 hasAuthorship W2073049420A5056318077 @default.
- W2073049420 hasAuthorship W2073049420A5070593854 @default.
- W2073049420 hasAuthorship W2073049420A5079187727 @default.
- W2073049420 hasBestOaLocation W20730494201 @default.
- W2073049420 hasConcept C181199279 @default.
- W2073049420 hasConcept C185592680 @default.
- W2073049420 hasConcept C2776151105 @default.
- W2073049420 hasConcept C2776231299 @default.
- W2073049420 hasConcept C2776359302 @default.
- W2073049420 hasConcept C2776672577 @default.
- W2073049420 hasConcept C2776932660 @default.
- W2073049420 hasConcept C2778102346 @default.
- W2073049420 hasConcept C2779470414 @default.
- W2073049420 hasConcept C2780829032 @default.
- W2073049420 hasConcept C2993539680 @default.
- W2073049420 hasConcept C42219234 @default.
- W2073049420 hasConcept C55493867 @default.
- W2073049420 hasConcept C71924100 @default.
- W2073049420 hasConcept C98274493 @default.
- W2073049420 hasConceptScore W2073049420C181199279 @default.
- W2073049420 hasConceptScore W2073049420C185592680 @default.
- W2073049420 hasConceptScore W2073049420C2776151105 @default.
- W2073049420 hasConceptScore W2073049420C2776231299 @default.
- W2073049420 hasConceptScore W2073049420C2776359302 @default.
- W2073049420 hasConceptScore W2073049420C2776672577 @default.
- W2073049420 hasConceptScore W2073049420C2776932660 @default.
- W2073049420 hasConceptScore W2073049420C2778102346 @default.
- W2073049420 hasConceptScore W2073049420C2779470414 @default.
- W2073049420 hasConceptScore W2073049420C2780829032 @default.
- W2073049420 hasConceptScore W2073049420C2993539680 @default.
- W2073049420 hasConceptScore W2073049420C42219234 @default.
- W2073049420 hasConceptScore W2073049420C55493867 @default.
- W2073049420 hasConceptScore W2073049420C71924100 @default.
- W2073049420 hasConceptScore W2073049420C98274493 @default.
- W2073049420 hasIssue "2" @default.
- W2073049420 hasLocation W20730494201 @default.
- W2073049420 hasLocation W20730494202 @default.
- W2073049420 hasOpenAccess W2073049420 @default.
- W2073049420 hasPrimaryLocation W20730494201 @default.
- W2073049420 hasRelatedWork W1565859929 @default.
- W2073049420 hasRelatedWork W1964838077 @default.
- W2073049420 hasRelatedWork W2012458878 @default.
- W2073049420 hasRelatedWork W2024977753 @default.
- W2073049420 hasRelatedWork W2067453753 @default.
- W2073049420 hasRelatedWork W2073049420 @default.
- W2073049420 hasRelatedWork W2076361895 @default.
- W2073049420 hasRelatedWork W2461819493 @default.