FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073069857> ?p ?o ?g. }

• W2073069857 endingPage "189" @default.
• W2073069857 startingPage "165" @default.
• W2073069857 abstract "A series of oligonucleotide primers are described which hybridize to conserved regions of influenza virus cDNA and prime DNA synthesis in Taq polymerase catalyzed amplification reactions (PCR). Primers were designed to hybridize as nested pairs and, following a two-step amplification, produce uniquely sized DNA fragments diagnostic for viral type and subtype. Influenza A and B matrix-protein genes and the influenza C haemagglutinin gene were targets for the type-specific primers. Subtype-specific primers targeted conserved sequences within the three haemagglutinin or two neuraminidase subtypes of different human influenza isolates. The utility of this method was demonstrated using computer search methods and by accurately amplifying DNA from a variety of influenza A, B, and C strains. Type-specific primer sets showed a broad type specificity and amplified DNA from viral strains of unknown sequence. Restriction mapping and DNA sequencing showed that fragments amplified in this manner derived from the input template, confirming the accuracy of the method and demonstrating how PCR can be used to quickly derive sufficient sequence information for analysis of viral relatedness. Subtyping primers were able to distinguish accurately between the three haemagglutinin (H1, H2, H3) and two neuraminidase (N1, N2) alleles of human influenza A isolates. Again DNA was amplified from viruses of unknown sequence confirming that most of these primer sets may prove useful as broad range subtyping reagents. In order to simplify the work associated with analysis of many samples, we have also devised a rapid method for the isolation of viral RNA and synthesis of cDNA. Using this 'mini-prep' technique, it is possible to detect, amplify, and identify picogram quantities of influenza virus in a single day, confirming that PCR provides a useful alternative to existing methods of influenza detection." @default.
• W2073069857 created "2016-06-24" @default.
• W2073069857 creator A5020926082 @default.
• W2073069857 creator A5049578859 @default.
• W2073069857 date "1991-06-01" @default.
• W2073069857 modified "2023-10-10" @default.
• W2073069857 title "Detection and identification of human influenza viruses by the polymerase chain reaction" @default.
• W2073069857 cites W1549268074 @default.
• W2073069857 cites W1551446447 @default.
• W2073069857 cites W1572817411 @default.
• W2073069857 cites W1577035872 @default.
• W2073069857 cites W1590528994 @default.
• W2073069857 cites W1608882086 @default.
• W2073069857 cites W1786990095 @default.
• W2073069857 cites W1907769169 @default.
• W2073069857 cites W1975810096 @default.
• W2073069857 cites W1984103323 @default.
• W2073069857 cites W1994298568 @default.
• W2073069857 cites W1996184929 @default.
• W2073069857 cites W1997085106 @default.
• W2073069857 cites W2004402674 @default.
• W2073069857 cites W2013514054 @default.
• W2073069857 cites W2022697750 @default.
• W2073069857 cites W2028622989 @default.
• W2073069857 cites W2031127338 @default.
• W2073069857 cites W2031342770 @default.
• W2073069857 cites W2034643905 @default.
• W2073069857 cites W2037614880 @default.
• W2073069857 cites W2040358152 @default.
• W2073069857 cites W2042928441 @default.
• W2073069857 cites W2048666161 @default.
• W2073069857 cites W2053108238 @default.
• W2073069857 cites W2058944751 @default.
• W2073069857 cites W2059278647 @default.
• W2073069857 cites W2062554335 @default.
• W2073069857 cites W2065351926 @default.
• W2073069857 cites W2070592586 @default.
• W2073069857 cites W2078421568 @default.
• W2073069857 cites W2079848331 @default.
• W2073069857 cites W2084872132 @default.
• W2073069857 cites W2090409312 @default.
• W2073069857 cites W2092868013 @default.
• W2073069857 cites W2095207427 @default.
• W2073069857 cites W2095438774 @default.
• W2073069857 cites W2110514306 @default.
• W2073069857 cites W2113940052 @default.
• W2073069857 cites W2118000074 @default.
• W2073069857 cites W2149170107 @default.
• W2073069857 cites W2154116964 @default.
• W2073069857 doi "https://doi.org/10.1016/0166-0934(91)90017-t" @default.
• W2073069857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1939505" @default.
• W2073069857 hasPublicationYear "1991" @default.
• W2073069857 type Work @default.
• W2073069857 sameAs 2073069857 @default.
• W2073069857 citedByCount "149" @default.
• W2073069857 countsByYear W20730698572012 @default.
• W2073069857 countsByYear W20730698572013 @default.
• W2073069857 countsByYear W20730698572014 @default.
• W2073069857 countsByYear W20730698572015 @default.
• W2073069857 countsByYear W20730698572016 @default.
• W2073069857 countsByYear W20730698572018 @default.
• W2073069857 countsByYear W20730698572019 @default.
• W2073069857 countsByYear W20730698572021 @default.
• W2073069857 countsByYear W20730698572022 @default.
• W2073069857 crossrefType "journal-article" @default.
• W2073069857 hasAuthorship W2073069857A5020926082 @default.
• W2073069857 hasAuthorship W2073069857A5049578859 @default.
• W2073069857 hasConcept C104317684 @default.
• W2073069857 hasConcept C129312508 @default.
• W2073069857 hasConcept C134164806 @default.
• W2073069857 hasConcept C153911025 @default.
• W2073069857 hasConcept C159047783 @default.
• W2073069857 hasConcept C178790620 @default.
• W2073069857 hasConcept C185592680 @default.
• W2073069857 hasConcept C187882448 @default.
• W2073069857 hasConcept C199360897 @default.
• W2073069857 hasConcept C200658500 @default.
• W2073069857 hasConcept C2522874641 @default.
• W2073069857 hasConcept C2777546802 @default.
• W2073069857 hasConcept C2777563447 @default.
• W2073069857 hasConcept C2781416681 @default.
• W2073069857 hasConcept C41008148 @default.
• W2073069857 hasConcept C49105822 @default.
• W2073069857 hasConcept C51679486 @default.
• W2073069857 hasConcept C52001828 @default.
• W2073069857 hasConcept C54355233 @default.
• W2073069857 hasConcept C552990157 @default.
• W2073069857 hasConcept C61053724 @default.
• W2073069857 hasConcept C82381507 @default.
• W2073069857 hasConcept C83852419 @default.
• W2073069857 hasConcept C86803240 @default.
• W2073069857 hasConcept C90583042 @default.
• W2073069857 hasConceptScore W2073069857C104317684 @default.
• W2073069857 hasConceptScore W2073069857C129312508 @default.
• W2073069857 hasConceptScore W2073069857C134164806 @default.
• W2073069857 hasConceptScore W2073069857C153911025 @default.
• W2073069857 hasConceptScore W2073069857C159047783 @default.
• W2073069857 hasConceptScore W2073069857C178790620 @default.

• next