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- W2073098190 abstract "Actin is the most abundant protein in the cytoplasm of most eukaryotic cells, which plays important roles in such processes as muscle contraction, cell division, morphogenesis, organelle positioning, and vesicle trafficking. In our previous study, we reported the expression method of recombinant beta-actin at high cell density in a shaking Erlenmeyer flask. In the insect cell culture at early stationary phase (1-1.2 x 107 cells per ml), additional nutrients or medium exchange at time of infection restored the expression level of beta-actin per cell equivalent to that in the cell culture at mid-logarithmic phase (1-2 x 106 cells per ml). Therefore, the volumetric yield of recombinant beta-actin, which was determined by the maximal cell density and the yield of recombinant protein per cell, was at least 6.5-fold higher than compared to the manufacturer's protocol. The present study further developed the expression method of recombinant beta-actin at the density of 1.75 x 107 cells per ml, which is ∼1.5-fold higher than the cell density at stationary phase. This method requires medium exchange plus additional nutrients and air ventilation after infection. The volumetric yield of recombinant beta-actin reached 200 mg per liter of insect cell culture. Here we report in detail the effective method for the production of recombinant beta-actin and the biological and the biochemical properties of recombinant beta-actin." @default.
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- W2073098190 date "2012-01-01" @default.
- W2073098190 modified "2023-09-27" @default.
- W2073098190 title "Expression of Recombinant Beta-Actin in the High-Density Insect Cell Culture" @default.
- W2073098190 doi "https://doi.org/10.1016/j.bpj.2011.11.2033" @default.
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