FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073113887> ?p ?o ?g. }

• W2073113887 endingPage "136" @default.
• W2073113887 startingPage "127" @default.
• W2073113887 abstract "To characterise fish haematopoietic stem/progenitor cells, it is necessary to develop a culture system that supports proliferation and differentiation of these cells. In the present study, we established cell lines from various tissues of carp (Cyprinus carpio) and ginbuna (Carassius auratus langsdorfii). By using these cell lines, we developed a culture system in which carp haematopoietic cells proliferated and were successively passaged. Cell lines from carp thymus (KoT), carp fin (KoF1) and ginbuna thymus (GTS6 and GTS9) were newly established. In addition to these cell lines, ginbuna fin (CFS) cell lines were also used as feeder layers. Kidney haematopoietic cells co-cultured with these feeder layers proliferated rapidly and were passaged over 20 times for more than 60 days. To characterise the proliferating cells, expression of marker genes for blood cell development were analysed. In the primary culture, marker genes for myeloid/erythroid progenitors (gata1), haematopoietic stem cells (gata2), neutrophils (mpx/mpo), B-cells (IgH) and T-cells (lck, TCRbeta and gata3) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). Expression of most of the genes disappeared after the third passage, only T-cell marker genes were highly expressed after passages. These results indicate that multiple blood cells developed in the primary culture and then T-cell lineages dominantly proliferated after several passages." @default.
• W2073113887 created "2016-06-24" @default.
• W2073113887 creator A5011994589 @default.
• W2073113887 creator A5020646606 @default.
• W2073113887 creator A5030337738 @default.
• W2073113887 creator A5048891278 @default.
• W2073113887 creator A5050307460 @default.
• W2073113887 creator A5055535201 @default.
• W2073113887 creator A5057607023 @default.
• W2073113887 creator A5061608163 @default.
• W2073113887 creator A5085004885 @default.
• W2073113887 date "2009-09-01" @default.
• W2073113887 modified "2023-10-12" @default.
• W2073113887 title "Co-culture of carp (Cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of T-cell lineages" @default.
• W2073113887 cites W183596108 @default.
• W2073113887 cites W1913056497 @default.
• W2073113887 cites W1963655585 @default.
• W2073113887 cites W1964934170 @default.
• W2073113887 cites W1983093800 @default.
• W2073113887 cites W1988385050 @default.
• W2073113887 cites W1988923477 @default.
• W2073113887 cites W1999708404 @default.
• W2073113887 cites W2010654438 @default.
• W2073113887 cites W2016833921 @default.
• W2073113887 cites W2020381360 @default.
• W2073113887 cites W2040217725 @default.
• W2073113887 cites W2041393747 @default.
• W2073113887 cites W2057921879 @default.
• W2073113887 cites W2061740109 @default.
• W2073113887 cites W2066565013 @default.
• W2073113887 cites W2068946617 @default.
• W2073113887 cites W2071410033 @default.
• W2073113887 cites W2085677599 @default.
• W2073113887 cites W2089250354 @default.
• W2073113887 cites W2093035843 @default.
• W2073113887 cites W2093851247 @default.
• W2073113887 cites W2098345433 @default.
• W2073113887 cites W2112528263 @default.
• W2073113887 cites W2132927231 @default.
• W2073113887 cites W2155048276 @default.
• W2073113887 cites W2172503413 @default.
• W2073113887 cites W2335485903 @default.
• W2073113887 cites W2409527434 @default.
• W2073113887 cites W2411288250 @default.
• W2073113887 cites W44606685 @default.
• W2073113887 doi "https://doi.org/10.1016/j.vetimm.2009.03.007" @default.
• W2073113887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19375173" @default.
• W2073113887 hasPublicationYear "2009" @default.
• W2073113887 type Work @default.
• W2073113887 sameAs 2073113887 @default.
• W2073113887 citedByCount "20" @default.
• W2073113887 countsByYear W20731138872012 @default.
• W2073113887 countsByYear W20731138872013 @default.
• W2073113887 countsByYear W20731138872015 @default.
• W2073113887 countsByYear W20731138872016 @default.
• W2073113887 countsByYear W20731138872017 @default.
• W2073113887 countsByYear W20731138872018 @default.
• W2073113887 countsByYear W20731138872019 @default.
• W2073113887 countsByYear W20731138872020 @default.
• W2073113887 countsByYear W20731138872022 @default.
• W2073113887 crossrefType "journal-article" @default.
• W2073113887 hasAuthorship W2073113887A5011994589 @default.
• W2073113887 hasAuthorship W2073113887A5020646606 @default.
• W2073113887 hasAuthorship W2073113887A5030337738 @default.
• W2073113887 hasAuthorship W2073113887A5048891278 @default.
• W2073113887 hasAuthorship W2073113887A5050307460 @default.
• W2073113887 hasAuthorship W2073113887A5055535201 @default.
• W2073113887 hasAuthorship W2073113887A5057607023 @default.
• W2073113887 hasAuthorship W2073113887A5061608163 @default.
• W2073113887 hasAuthorship W2073113887A5085004885 @default.
• W2073113887 hasConcept C109159458 @default.
• W2073113887 hasConcept C153911025 @default.
• W2073113887 hasConcept C201750760 @default.
• W2073113887 hasConcept C203014093 @default.
• W2073113887 hasConcept C2778891732 @default.
• W2073113887 hasConcept C2779282312 @default.
• W2073113887 hasConcept C28328180 @default.
• W2073113887 hasConcept C2909208804 @default.
• W2073113887 hasConcept C505870484 @default.
• W2073113887 hasConcept C54355233 @default.
• W2073113887 hasConcept C81885089 @default.
• W2073113887 hasConcept C86803240 @default.
• W2073113887 hasConcept C95444343 @default.
• W2073113887 hasConceptScore W2073113887C109159458 @default.
• W2073113887 hasConceptScore W2073113887C153911025 @default.
• W2073113887 hasConceptScore W2073113887C201750760 @default.
• W2073113887 hasConceptScore W2073113887C203014093 @default.
• W2073113887 hasConceptScore W2073113887C2778891732 @default.
• W2073113887 hasConceptScore W2073113887C2779282312 @default.
• W2073113887 hasConceptScore W2073113887C28328180 @default.
• W2073113887 hasConceptScore W2073113887C2909208804 @default.
• W2073113887 hasConceptScore W2073113887C505870484 @default.
• W2073113887 hasConceptScore W2073113887C54355233 @default.
• W2073113887 hasConceptScore W2073113887C81885089 @default.
• W2073113887 hasConceptScore W2073113887C86803240 @default.
• W2073113887 hasConceptScore W2073113887C95444343 @default.
• W2073113887 hasIssue "1-2" @default.
• W2073113887 hasLocation W20731138871 @default.

• next