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• W2073169008 endingPage "033018" @default.
• W2073169008 startingPage "033018" @default.
• W2073169008 abstract "This work reports our efforts on developing simple-to-use microfluidic devices for point-of-care diagnostic applications with recent extensions that include the trapping of microbeads using dielectrophoresis (DEP) and the modulation of the liquid flow using integrated microheaters. DEP serves the purpose of trapping microbeads coated with receptors and analytes for detection of a fluorescent signal. The microheater is actuated once the chip is filled by capillarity, creating an evaporation-induced flow tuned according to assay conditions. The chips are composed of a glass substrate patterned with 50-nm-thick Pd electrodes and microfluidic structures made using a 20-μm-thick dry-film resist (DFR). Chips are covered/sealed by low temperature (50°C) lamination of a 50-μm-thick DFR layer having excellent optical and mechanical properties. To separate cleaned and sealed chips from the wafer, we used an effective chip singulation technique which we informally call the “chip-olate” process. In the experimental section, we first studied dielectrophoretic trapping of 10-μm beads for flow rates ranging from 80 pL s−1 to 2.5 nL s−1 that are generated by an external syringe pump. Then, we characterized the embedded microheater in DFR-covered chips. Flow rates as high as 8 nL s−1 were generated by evaporation-induced flow when the heater was biased by 10 V, corresponding to 270-mW power. Finally, DEP-based trapping and fluorescent detection of functionalized beads were demonstrated as the flow was generated by evaporation-induced flow after the microfluidic structures were filled by capillarity." @default.
• W2073169008 created "2016-06-24" @default.
• W2073169008 creator A5033412633 @default.
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• W2073169008 date "2014-09-23" @default.
• W2073169008 modified "2023-09-23" @default.
• W2073169008 title "Capillary-driven microfluidic chips with evaporation-induced flow control and dielectrophoretic microbead trapping" @default.
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• W2073169008 doi "https://doi.org/10.1117/1.jmm.13.3.033018" @default.
• W2073169008 hasPublicationYear "2014" @default.
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