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- W2073188468 abstract "Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluorescent and electron microscopic methods. Both Glu- and Tyr-tubulins were detected by immunoblot analysis. Immunofluorescent microscopy indicated that the alpha-tubulin isotypes are concentrated in different regions of permeabilized cells: Glu-tubulin is located primarily in cirri, membranelles, and surrounding the macro- and micronuclei. Tyr-tubulin is principally at the bases of cirri and membranelles. This differential distribution of alpha-tubulin isotypes is discussed in terms of current concepts concerning the correlation of tubulin post-translational modifications to microtubule stability. Confocal immunofluorescent imaging was of critical importance in clearly differentiating the Glu-tubulin isotype surrounding the macro- and micronuclei from a brilliantly fluorescent environment originating from cytoskeletal structures. In conjunction with conventional and stereo-electron microscopy, confocal optical microscopy provided convincing evidence for a basket of microtubules surrounding both nuclei." @default.
- W2073188468 created "2016-06-24" @default.
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- W2073188468 date "1989-01-01" @default.
- W2073188468 modified "2023-09-26" @default.
- W2073188468 title "Differential distribution of α-tubulin isotypes in Euplotes eurystomus determined by confocal immunofluorescence microscopy**" @default.
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- W2073188468 doi "https://doi.org/10.1111/j.1768-322x.1989.tb00840.x" @default.
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