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• W2073199563 abstract "Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides, siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to microg range) to living neurons in a reasonable period of time." @default.
• W2073199563 created "2016-06-24" @default.
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• W2073199563 date "2008-10-01" @default.
• W2073199563 modified "2023-09-25" @default.
• W2073199563 title "Microinjection into cultured hippocampal neurons: A straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies" @default.
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• W2073199563 doi "https://doi.org/10.1016/j.jneumeth.2008.08.004" @default.
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