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- W2073201322 abstract "In shotgun proteomics, protein mixtures are proteolytically digested before tandem mass spectrometry (MS/MS) analysis. Biological samples are generally characterized by a very high complexity, therefore a step of peptides fractionation before the MS analysis is essential. This passage reduces the sample complexity and increases its compatibility with the sampling performance of the instrument. Among all the existing approaches for peptide fractionation, isoelectric focusing has several peculiarities that are theoretically known but practically rarely exploited by the proteomics community. The main aim of this review is to draw the readers’ attention to these unique qualities, which are not accessible with other common approaches, and that represent important tools to increase confidence in the identification of proteins and some post-translational modifications. The general characteristics of different methods to perform peptide isoelectric focusing with natural and artificial pH gradients, the existing instrumentation, and the informatics tools available for isoelectric point calculation are also critically described. Finally, we give some general conclusions on this strategy, underlying its principal limitations." @default.
- W2073201322 created "2016-06-24" @default.
- W2073201322 creator A5007177776 @default.
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- W2073201322 creator A5060623512 @default.
- W2073201322 creator A5071985025 @default.
- W2073201322 creator A5088748843 @default.
- W2073201322 date "2013-06-01" @default.
- W2073201322 modified "2023-10-07" @default.
- W2073201322 title "Pros and cons of peptide isolectric focusing in shotgun proteomics" @default.
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- W2073201322 doi "https://doi.org/10.1016/j.chroma.2013.03.073" @default.
- W2073201322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23639126" @default.
- W2073201322 hasPublicationYear "2013" @default.
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