Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073245847> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2073245847 endingPage "1255???1264" @default.
- W2073245847 startingPage "1255???1264" @default.
- W2073245847 abstract "Insulin may influence platelet function by modulating platelet responsiveness to vasoactive agonists. In essential hypertension platelet hyperactivity might be related to altered modulation by insulin. The present study examined the effects of physiological concentrations of insulin on agonist-stimulated platelet responses [free calcium concentration, intracellular pH (pHi) and thrombin-induced aggregation] in platelets from 30 normotensive subjects and 53 untreated essential hypertensive patients.Platelet free calcium concentration and pHi were measured spectrofluorometrically using specific fluorescent dyes [Fura-2/acetoxymethyl ester and 2',7'-bis-(carboxyethyl)-5(6)-carboxyfluorescein/acetoxymethyl ester, respectively] in unstimulated and in angiotensin II (Ang II; 1 nmol/l)- and endothelin-1 (1 nmol/l)-stimulated platelets that had been pre-exposed to insulin (70 microU/ml). Aggregatory responses were measured turbidometrically in platelets stimulated by thrombin (0.05 U/ml), alone or in combination with angiotensin II or endothelin-1 in the absence and presence of insulin pre-incubation.Blood pressure and serum glucose and insulin levels were significantly elevated in the hypertensive patients. Basal and agonist-stimulated free calcium concentration, pHi and aggregation were significantly higher in the hypertensive than in the normotensive group. In platelets pre-exposed to insulin for 5 min, agonist-induced responses were significantly reduced (by 40-60%) in the normotensive group but not in the hypertensive group. Serum insulin was positively correlated with Ang II-stimulated cytosolic free calcium concentration (r = 0.47, P < 0.01) and pHi (r = 0.26, P = 0.047) in platelets pre-exposed to insulin.This study demonstrates that insulin inhibits Ang II- and endothelin-1-stimulated platelet free calcium concentration and pHi, and thrombin-induced aggregation. In hypertension these insulin-related inhibitory effects are blunted. Attenuated inhibition of agonist-stimulated platelet responses by insulin could be a manifestation of peripheral insulin resistance which may contribute to platelet hyperactivity in essential hypertension." @default.
- W2073245847 created "2016-06-24" @default.
- W2073245847 creator A5020577597 @default.
- W2073245847 creator A5046910928 @default.
- W2073245847 date "1994-11-01" @default.
- W2073245847 modified "2023-10-15" @default.
- W2073245847 title "Blunted inhibition by insulin of agonist-stimulated calcium, pH and aggregatory responses in platelets from hypertensive patients" @default.
- W2073245847 doi "https://doi.org/10.1097/00004872-199411000-00008" @default.
- W2073245847 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7868873" @default.
- W2073245847 hasPublicationYear "1994" @default.
- W2073245847 type Work @default.
- W2073245847 sameAs 2073245847 @default.
- W2073245847 citedByCount "16" @default.
- W2073245847 countsByYear W20732458472013 @default.
- W2073245847 countsByYear W20732458472016 @default.
- W2073245847 countsByYear W20732458472023 @default.
- W2073245847 crossrefType "journal-article" @default.
- W2073245847 hasAuthorship W2073245847A5020577597 @default.
- W2073245847 hasAuthorship W2073245847A5046910928 @default.
- W2073245847 hasConcept C126322002 @default.
- W2073245847 hasConcept C134018914 @default.
- W2073245847 hasConcept C144980905 @default.
- W2073245847 hasConcept C170493617 @default.
- W2073245847 hasConcept C2778938600 @default.
- W2073245847 hasConcept C2779306644 @default.
- W2073245847 hasConcept C2908929049 @default.
- W2073245847 hasConcept C519063684 @default.
- W2073245847 hasConcept C71924100 @default.
- W2073245847 hasConcept C84393581 @default.
- W2073245847 hasConcept C89560881 @default.
- W2073245847 hasConceptScore W2073245847C126322002 @default.
- W2073245847 hasConceptScore W2073245847C134018914 @default.
- W2073245847 hasConceptScore W2073245847C144980905 @default.
- W2073245847 hasConceptScore W2073245847C170493617 @default.
- W2073245847 hasConceptScore W2073245847C2778938600 @default.
- W2073245847 hasConceptScore W2073245847C2779306644 @default.
- W2073245847 hasConceptScore W2073245847C2908929049 @default.
- W2073245847 hasConceptScore W2073245847C519063684 @default.
- W2073245847 hasConceptScore W2073245847C71924100 @default.
- W2073245847 hasConceptScore W2073245847C84393581 @default.
- W2073245847 hasConceptScore W2073245847C89560881 @default.
- W2073245847 hasIssue "11" @default.
- W2073245847 hasLocation W20732458471 @default.
- W2073245847 hasLocation W20732458472 @default.
- W2073245847 hasOpenAccess W2073245847 @default.
- W2073245847 hasPrimaryLocation W20732458471 @default.
- W2073245847 hasRelatedWork W13788639 @default.
- W2073245847 hasRelatedWork W1499732058 @default.
- W2073245847 hasRelatedWork W1982913438 @default.
- W2073245847 hasRelatedWork W1998274488 @default.
- W2073245847 hasRelatedWork W2048213734 @default.
- W2073245847 hasRelatedWork W2082094685 @default.
- W2073245847 hasRelatedWork W2083197627 @default.
- W2073245847 hasRelatedWork W2418625451 @default.
- W2073245847 hasRelatedWork W3198306613 @default.
- W2073245847 hasRelatedWork W4242457046 @default.
- W2073245847 hasVolume "12" @default.
- W2073245847 isParatext "false" @default.
- W2073245847 isRetracted "false" @default.
- W2073245847 magId "2073245847" @default.
- W2073245847 workType "article" @default.