Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073264243> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2073264243 endingPage "18" @default.
- W2073264243 startingPage "13" @default.
- W2073264243 abstract "The aminoglycoside 3'-phosphotransferase type VIII (APHVIII) encoding gene (aphVIII) from Streptomyces rimosus was introduced by glass-bead high-efficiency transformation into the nuclear genome of green unicellular alga Chlamydomonas reinhardtii for induction of transformants resistant to aminoglycoside antibiotics. The aphVIII structural sequence was flanked by S. rimosus regulatory sequences which failed to direct expression in C. reinhardtii. The pSU937 plasmid containing these sequences was able to transform C. reinhardtii strain cw15 arg7-8 mt+ for paromomycin resistance (PmR) at a frequency (1.3-1.9) x 10(-7), probably as a result of in vivo gene fusion and expression of the aphVIII gene from regulatory elements of nuclear DNA. Evidence for the real C. reinhardtii transformation includes blot-hybridization with a probe specific for aphVIII and demonstration of APHVIII enzyme activity in crude cell extracts of transformants. Integrated Streptomyces DNA sequences, APHVIII enzyme activity and the aminoglycoside-resistance phenotype were stable through mitosis in the presence and absence of selection. The PmR phenotype was inherited by the meiotic progeny of transformants from crosses with wild-type strains." @default.
- W2073264243 created "2016-06-24" @default.
- W2073264243 creator A5002581889 @default.
- W2073264243 creator A5021903459 @default.
- W2073264243 creator A5027724136 @default.
- W2073264243 creator A5034754203 @default.
- W2073264243 creator A5049180298 @default.
- W2073264243 creator A5075460693 @default.
- W2073264243 date "1996-11-01" @default.
- W2073264243 modified "2023-10-14" @default.
- W2073264243 title "Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker" @default.
- W2073264243 cites W1601471170 @default.
- W2073264243 cites W1775091795 @default.
- W2073264243 cites W1781554643 @default.
- W2073264243 cites W1907935562 @default.
- W2073264243 cites W1935281316 @default.
- W2073264243 cites W1973454389 @default.
- W2073264243 cites W2012019347 @default.
- W2073264243 cites W2013512070 @default.
- W2073264243 cites W2065433789 @default.
- W2073264243 cites W2066352920 @default.
- W2073264243 cites W2130496637 @default.
- W2073264243 doi "https://doi.org/10.1016/s0378-1119(96)00384-8" @default.
- W2073264243 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8973302" @default.
- W2073264243 hasPublicationYear "1996" @default.
- W2073264243 type Work @default.
- W2073264243 sameAs 2073264243 @default.
- W2073264243 citedByCount "56" @default.
- W2073264243 countsByYear W20732642432012 @default.
- W2073264243 countsByYear W20732642432013 @default.
- W2073264243 countsByYear W20732642432014 @default.
- W2073264243 countsByYear W20732642432015 @default.
- W2073264243 countsByYear W20732642432016 @default.
- W2073264243 countsByYear W20732642432017 @default.
- W2073264243 countsByYear W20732642432018 @default.
- W2073264243 countsByYear W20732642432019 @default.
- W2073264243 countsByYear W20732642432020 @default.
- W2073264243 countsByYear W20732642432021 @default.
- W2073264243 countsByYear W20732642432022 @default.
- W2073264243 countsByYear W20732642432023 @default.
- W2073264243 crossrefType "journal-article" @default.
- W2073264243 hasAuthorship W2073264243A5002581889 @default.
- W2073264243 hasAuthorship W2073264243A5021903459 @default.
- W2073264243 hasAuthorship W2073264243A5027724136 @default.
- W2073264243 hasAuthorship W2073264243A5034754203 @default.
- W2073264243 hasAuthorship W2073264243A5049180298 @default.
- W2073264243 hasAuthorship W2073264243A5075460693 @default.
- W2073264243 hasConcept C104317684 @default.
- W2073264243 hasConcept C141231307 @default.
- W2073264243 hasConcept C143065580 @default.
- W2073264243 hasConcept C153911025 @default.
- W2073264243 hasConcept C204241405 @default.
- W2073264243 hasConcept C20750100 @default.
- W2073264243 hasConcept C22744801 @default.
- W2073264243 hasConcept C2776580309 @default.
- W2073264243 hasConcept C2777727519 @default.
- W2073264243 hasConcept C2779920670 @default.
- W2073264243 hasConcept C523546767 @default.
- W2073264243 hasConcept C54355233 @default.
- W2073264243 hasConcept C86803240 @default.
- W2073264243 hasConceptScore W2073264243C104317684 @default.
- W2073264243 hasConceptScore W2073264243C141231307 @default.
- W2073264243 hasConceptScore W2073264243C143065580 @default.
- W2073264243 hasConceptScore W2073264243C153911025 @default.
- W2073264243 hasConceptScore W2073264243C204241405 @default.
- W2073264243 hasConceptScore W2073264243C20750100 @default.
- W2073264243 hasConceptScore W2073264243C22744801 @default.
- W2073264243 hasConceptScore W2073264243C2776580309 @default.
- W2073264243 hasConceptScore W2073264243C2777727519 @default.
- W2073264243 hasConceptScore W2073264243C2779920670 @default.
- W2073264243 hasConceptScore W2073264243C523546767 @default.
- W2073264243 hasConceptScore W2073264243C54355233 @default.
- W2073264243 hasConceptScore W2073264243C86803240 @default.
- W2073264243 hasIssue "1-2" @default.
- W2073264243 hasLocation W20732642431 @default.
- W2073264243 hasLocation W20732642432 @default.
- W2073264243 hasOpenAccess W2073264243 @default.
- W2073264243 hasPrimaryLocation W20732642431 @default.
- W2073264243 hasRelatedWork W1964404589 @default.
- W2073264243 hasRelatedWork W1999022018 @default.
- W2073264243 hasRelatedWork W2041808497 @default.
- W2073264243 hasRelatedWork W2144145338 @default.
- W2073264243 hasRelatedWork W230946013 @default.
- W2073264243 hasRelatedWork W2391646789 @default.
- W2073264243 hasRelatedWork W2529233322 @default.
- W2073264243 hasRelatedWork W3092054307 @default.
- W2073264243 hasRelatedWork W4245886947 @default.
- W2073264243 hasRelatedWork W4385658311 @default.
- W2073264243 hasVolume "181" @default.
- W2073264243 isParatext "false" @default.
- W2073264243 isRetracted "false" @default.
- W2073264243 magId "2073264243" @default.
- W2073264243 workType "article" @default.