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- W2073284772 abstract "Aminoacyl-tRNA synthetases extracted from healthy and crown gall tumor tissues (induced by Agrobacterium tumefaciens strain B6) from Nicotiana tabacum (strain Wisconsin 38) grown in vitro, showed the same ability to charge Phaseolus vulgaris tRNA, for all the 15 amino acids tested. For each amino acid, optimal charging conditions (enzyme concentration, Mg2+/ATP ratios, K+ ion effects) have been determined with Phaseolus vulgaris tRNA and were found to be the same whether aminoacyl-tRNA synthetases from healthy or tumor tissues were used. In each case, valyl- and glutamyl-tRNA synthetases were very sensitive to an excess of Mg2+ and K+ ions. Although tRNA's extracted from healthy and tumor tissues gave the same electrophoretic patterns, charging levels obtained with turner tRNAs were generally 45% higher than those obtained with tRNA's from healthy tissues." @default.
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- W2073284772 date "1975-02-01" @default.
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- W2073284772 title "Characterization of the tRNA and aminoacyl-tRNA synthetases of healthy and tumorous callus tissues from Nicotiana tabacum" @default.
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- W2073284772 doi "https://doi.org/10.1016/0031-9422(75)85098-9" @default.
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