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• W2073295780 startingPage "348" @default.
• W2073295780 abstract "The purpose of this study was to determine the direct effects of lipopolysaccharide (LPS) on osteoclastogenesis and to assess the ability of calcium hydroxide-Ca(OH)(2)-to inhibit the osteoclast formation stimulated by LPS.RAW 264.7 cells were cultured with 50 ng/mL recombinant receptor activator of NF-kappaB ligand (RANKL) for 72 hours. RANKL was then removed, and the cells were treated with 0, 1, 10, or 100 ng/mL of LPS, Ca(OH)(2)-treated LPS, or 50 ng/mL of RANKL as a positive control for an additional 48 hours. Cells were fixed and stained with fluorescein isothiocyanate-conjugated phalloidin to detect actin ring formation, and histochemistry was performed to detect multinucleated cells expressing tartrate-resistant acid phosphatase activity.LPS induced osteoclast-like cell (OCL) formation in a dose-dependent manner when osteoclast precursor RAW 264.7 cells were pretreated for 72 hours with RANKL. Ca(OH)(2) significantly inhibited the ability of LPS to stimulate OCL formation.This study shows that LPS directly stimulates OCL formation. The detoxification of LPS by treatment with Ca(OH)(2) significantly reduced its ability to trigger the differentiation of OCLs." @default.
• W2073295780 created "2016-06-24" @default.
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• W2073295780 date "2003-03-01" @default.
• W2073295780 modified "2023-09-26" @default.
• W2073295780 title "Calcium hydroxide reduces lipopolysaccharide-stimulated osteoclast formation" @default.
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• W2073295780 doi "https://doi.org/10.1067/moe.2003.18" @default.
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